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在不同保存温度下，探究灵芝多糖在獭兔精液稀释液中的适宜添加浓度以及保存效果。在稀释液中加不同浓度（0.00 mg/mL、0.05 mg/mL、0.10 mg/mL、0.15 mg/mL）的灵芝多糖，将獭兔精液分别保存于恒温（37℃）、常温（20℃）和低温（4℃）环境中0 h、2 h、4 h、6 h后，对其精子活率、畸形率、质膜完整性、顶体完整性以及抗氧化指标包括超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-PX）、过氧化氢酶（CAT）活性、丙二醛（MDA）含量及总抗氧化能力（T-AOC）进行检测。结果显示，当添加0.10 mg/mL灵芝多糖时，低温和常温保存后獭兔精子的活率、顶体完整性和质膜完整性均显著提高（P＜0.05），畸形率显著降低（P＜0.05）；当添加0.15 mg/mL灵芝多糖时，恒温保存后獭兔精子的活率、顶体完整性和质膜完整性均显著提高（P＜0.05），畸形率显著降低（P＜0.05）；在恒温保存后，0.15 mg/mL添加组的SOD、GSH-PX、CAT活性和T-AOC显著提高（P＜0.05），MDA含量显著降低（P＜0.05）；在常温和低温保存后，0.10 mg/mL添加组的SOD、GSH-PX、CAT活性和T-AOC显著提高（P＜0.05），MDA含量显著降低（P＜0.05）。研究表明，在獭兔精液保存稀释液中添加0.10 mg/mL的灵芝后多糖后，其低温保存效果最好，建议在獭兔人工授精实践中可作为参考。
Abstract：This study aimed to investigate the optimal concentration of Ganoderma lucidum polysaccharides (GLP) in semen extenders for Rex rabbits and its preserving efficacy under different storage temperatures. Semen samples were supplemented with varying GLP concentrations (0.00， 0.05， 0.10， and 0.15 mg/mL) and stored under constant temperature (37℃)， room temperature (20℃)， and low temperature (4℃) conditions. Evaluations were conducted at 0， 2， 4， and 6 hours post-preservation， assessing sperm motility， abnormality rate， plasma membrane integrity， acrosome integrity， and antioxidant indices including superoxide dismutase (SOD)， glutathione peroxidase (GSH-Px)， catalase (CAT) activities， malondialdehyde (MDA) content， and total antioxidant capacity (T-AOC). Results indicated that at 0.10?mg/mL GLP， sperm motility， acrosome integrity， and plasma membrane integrity significantly improved (P?
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基本信息:
中图分类号:S829.1
引用信息:
[1]李思源,刘波,郭通斌,等.添加灵芝多糖对獭兔精液不同温度保存效果的影响[J].经济动物学报().
基金信息:
国家自然科学基金项目（31560634）
2026-03-23
2026-03-23
2026-03-23