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为研究圈养环境与野生环境下的东北林蛙肠道细菌群落结构和功能的差异，于2023年4月-5月采集辽宁地区野生东北林蛙12只，圈养东北林蛙12只，分别取样十二指肠、小肠和大肠3个部位，合计72个样本，采用16 S rDNA高通量测序技术鉴定菌群结构，采用Alpha多样性分析、Beta多样性分析、Anosim分析和聚类分析方法比较两组间菌群差异，结果如下：（1）野生组林蛙肠道优势菌门为变形菌门（Proteobacteria）、厚壁菌门（Firmicutes）和放线菌门（Actinobacteria），优势菌属为不动杆菌属（Acinetobacter）、柠檬酸杆菌属（Citrobacter）和鞘氨醇杆菌属（Sphingobacterium）；圈养组优势菌门为变形菌门、厚壁菌门和拟杆菌门（Bacteroidetes），优势菌属为黄金杆菌属（Chryseobacterium）、希瓦氏菌属（Shewanella）和黄杆菌属（Flavobacterium）。（2）在东北林蛙全肠道中，变形菌门、放线菌门和拟杆菌门均为相对丰度较高的菌门，相对丰度均为野生低于圈养。（3）野生和圈养组东北林蛙整体肠道细菌alpha多样性和beta多样性差异均显著；对野生和圈养东北林蛙的相同肠道部位之间的多样性进行比较，除小肠部位的alpha多样性和直肠部位的beta多样性差异显著外，其余均不显著。本研究结果为东北林蛙的保护工作和健康养殖提供科学依据。
Abstract：To investigate the differences in the structure and function of gut bacterial communities between captive and wild Rana dybowskii， 12 wild Rana dybowskii and 12 captive Rana dybowskii were collected from April to May 2023 in Liaoning Province. Samples were taken from the duodenum， small intestine， and large intestine， totaling 72 samples. The microbial community structure was identified using high-throughput sequencing technology with 16S rDNA. Alpha diversity analysis， Beta diversity analysis， Anosim analysis， and cluster analysis were used to compare the differences in microbial communities between the two groups. The results are as follows: (1) The dominant phyla in the intestinal tract of wild Rana dybowskii are Proteobacteria， Firmicutes， and Actinobacteria， while the dominant genera are Acinetobacter， Citrobacter， and Sphingobacter; The dominant phyla in the captive group are Proteobacteria， Firmicutes， and Bacteroidetes， while the dominant genera are Chryseobacteria， Shewanella， and Flavobacteria. (2) In the entire gut of Rana dybowskii， the phyla Proteobacteria， Actinobacteria， and Bacteroidetes are relatively abundant， with relative abundance lower in the wild than in captivity. (3) There were significant differences in alpha and beta diversity of gut bacteria between wild and captive groups of Rana dybowskii; Comparing the diversity of the same intestinal parts between wild and captive Rana dybowskii， except for significant differences in alpha diversity of the small intestine and beta diversity of the rectum， all others were not significant. The results of this study provide scientific basis for the protection and healthy breeding of Rana dybowskii.
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基本信息:
中图分类号:S917.4
引用信息:
[1]张诗琪,贺晨,花语璐,等.辽宁圈养和野生东北林蛙肠道细菌多样性比较[J].经济动物学报().
基金信息:
辽宁省农业农村厅专项（202401）
2025-01-21
2025-01-21
2025-01-21