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牛胸腺肽是一类天然活性物质，具有抗病毒活性，但对IBRV是否有抑制作用尚未见报道。本研究在建立兔感染IBRV模型基础上，从体外培养细胞和兔体内两方面探究牛胸腺肽对IBRV的抑制作用及防治效果。。体外试验结果表明，牛胸腺肽在胞内胞外以及对病毒的入侵和复制过程有抑制作用，胞外作用9 h时可以达到最佳抑制效果，病毒拷贝数极显著低于病毒对照组(P<0.001)；胞内作用48 h，病毒拷贝数显著低于病毒对照组(P<0.05)，达到显著抑制效果。以1.0×104.59 TCID50/mL剂量接种日本大耳白兔，可使其出现典型临床症状和剖检病理变化。在治疗试验中，口服牛胸腺肽2 mL剂量组在体温变化等临床症状方面与病毒对照组、利巴韦林组相比差异均显著；临床剖检的肺脏以及组织病理切片也较病毒对照组有明显减轻，脏器病毒载量极显著低于病毒对照组和利巴韦林组(P<0.001)；预防试验中疫苗组的临床症状和剖检脏器病理变化比病毒对照组及牛胸腺肽组都有明显减轻，脏器病毒载量极显著低于病毒对照组和牛胸腺肽组(P<0.001)。本研究通过体外试验证明了牛胸腺肽对IBRV具有抑制效果，家兔感染模型证明牛胸腺肽对接毒家兔有治疗和预防效果，治疗效果优于利巴韦林，预防效果不及商品化疫苗。本研究结果为临床防治牛传染性鼻气管炎提供了候选药物。
Abstract：Bovine thymosin is a kind of natural active substance with antiviral activity. However， there are no reports on its inhibitory effect on IBRV. In this study， based on the establishment of a rabbit model infected with IBRV， the inhibitory effect and preventive and therapeutic effects of bovine thymosin on IBRV were investigated from both in vitro cultured cells and in vivo rabbit models. The in vitro test results showed that bovine thymosin had inhibitory effects on the virus both inside and outside the cells， and the best inhibitory effect was achieved after 9 hours of extracellular action， with the viral copy number being significantly lower than that of the virus control group (P < 0.001); after 48 hours of intracellular action， the viral copy number was significantly lower than that of the virus control group (P < 0.05)， achieving a significant inhibitory effect. Japanese white rabbits were inoculated with a dose of 1.0×104.59 TCID50/mL， which could cause typical clinical symptoms and pathological changes in post-mortem examination. In the treatment test， the 2 mL oral dose group of bovine thymosin showed significant differences in clinical symptoms such as body temperature changes compared with the virus control group and the ribavirin group; the pathological changes in the lungs and tissue sections in clinical post-mortem examination were also significantly alleviated compared with the virus control group， and the viral load in the organs was significantly lower than that of the virus control group and the ribavirin group (P < 0.001). In the prevention test， the clinical symptoms and post-mortem pathological changes of the organs in the vaccine group were significantly alleviated compared with the virus control group and the bovine thymosin group， and the viral load in the organs was significantly lower than that of the virus control group and the bovine thymosin group (P < 0.001). This study proved that bovine thymosin has an inhibitory effect on IBRV through in vitro tests， and the rabbit infection model demonstrated that bovine thymosin has therapeutic and preventive effects on infected rabbits. The therapeutic effect is better than that of ribavirin， but the preventive effect is not as good as commercial vaccines. The results of this study provide candidate drugs for the clinical prevention and treatment of infectious bovine rhinotracheitis.
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基本信息:
中图分类号:S858.23
引用信息:
[1]凤焱,于雪彤,刘云霞,等.基于细胞培养和兔感染模型初步评价牛胸腺肽对牛传染性鼻气管炎病毒抑制及防治效果[J].经济动物学报().
基金信息:
吉林省科学发展计划项目（20210202101NC）
2025-07-11
2025-07-11
2025-07-11