经济动物学报

探索哈蟆油酶解肽对糖皮质激素诱导骨质疏松小鼠的治疗作用。将6周龄的36只KM雌性小鼠，随机分为空白对照组（con）、模型对照组（mod）、阳性对照组、哈蟆油酶解肽高剂量组（H）、哈蟆油酶解肽中剂量组（M）、哈蟆油酶解肽低剂量组（L），共6组，每组6只。试验组分别给予相应的药物灌胃及臀肌注射，连续给药6周。试验结束，采血分离血清，并将动物处死，取股骨并制成股骨病理切片，常规HE染色，用显微镜在100倍下观察骨组织形态和结构并拍照记录切片。测定血清中AKP和TRAP的含量。使用双能X射线骨密度仪检查其骨密度值，使用ImageJ图像处理软件和SPSS19.0统计学软件分析数据。结果 与空白对照组比较，哈蟆油酶解肽组的小鼠AKP酶活增加，TRAP含量增加（P＜0.001），骨小梁结构稀疏，骨密度减少，骨髓减少；与模型组相比，哈蟆油酶解肽组的小鼠AKP酶活降低（P＜0.01），TRAP含量降低（P＜0.001），骨小梁丢失的程度有所改善，其中H组酶解肽的保护效果最好。与空白对照组相比，模型对照组小鼠股骨小梁排列稀疏、变细、数目减少、间距加宽，股骨小梁面积比例降低，提示造模成功；与模型对照组相比，哈蟆油酶解肽组小鼠股骨小梁较饱满，排列较有序，数目及粗细适中，股骨小梁面积比例高于模型对照组。哈蟆油酶解肽对糖皮质激素诱导骨质疏松具有治疗作用。

To investigate the therapeutic effect of enzymatic hydrolyzed peptides from Oviductus ranae on glucocorticoid-induced osteoporosis of mice. A total of 36 KM female mice aged 6 weeks were weighed and randomly divided into blank control group (con)， model control group (mod)， positive control group， high-dose peptide group (H)， medium-dose peptide group (M)， and low-dose peptide group (L)， a total of 5 groups， with 6 mice in each group. The corresponding drugs were given by gavage and gluteal muscle injection， which were continuously administered for 6 weeks， and then they were sacrificed， and the femur was taken and made into femoral pathological sections. Conventional HE stained， bone tissue morphology and structure were observed with a microscope at 100x and the sections were recorded， the bone mineral density value was checked by dual-energy X-ray bone densitometer， and the data were analyzed by ImageJ image processing software and SPSS19.0 statistical software. Results Compared with the blank control group， the activity of AKPase was increased， the content of TRAP increased (P<0.001)， the trabecular bone structure was sparse， the bone mineral density was reduced， and the bone marrow was reduced， and compared with the model group， the AKP enzyme activity was decreased (P<0.01)， the TRAP content was decreased (P<0.001)， and the degree of trabecular bone loss was improved. Compared with the blank control group， the trabecular femur arrangement in the model control group was sparse， thinner， the number decreased， the spacing widened， and the proportion of trabecular femur area decreased， indicating that the modeling was successful. Compared with the model control group， the trabecular femurs in the toad oil hydrolyzed peptide group were fuller， more orderly， with moderate number and thickness， and the proportion of trabecular femur area was higher than that of the model control group. Python oil hydrolyzed peptide has a therapeutic effect on glucocorticoid-induced osteoporosis.