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为研究饲料中牛磺脱氧胆酸（Taurousodeoxycholic acid，TUDCA）对泥鳅（Misgurnus anguillicaudatus）生长性能、免疫功能、肝胰脏健康及胆汁酸代谢的影响。本试验在基础饲料中分别添加0、100、200、300和400 mg/kg的TUDCA，即对照（CON）组、TU100、TU200、TU300和TU400组，进行为期56 d的饲养试验，测量生长性能指标并对免疫功能指标、免疫功能相关基因及胆汁酸代谢相关基因的表达进行检测。结果显示，与CON组相比，各试验组终末体质量均显著提升（P<0.05），TU200、TU300与TU400组泥鳅增重率（WG）和特定生长率（SGR）均显著升高（P<0.05）；TU300组蛋白质效率比（PER）和饲料转化率（FCR）得到显著提升（P<0.05）；TU200、TU300和TU400组泥鳅血清中补体3（C3）、补体4（C4）与免疫球蛋白M（IgM）含量显著升高（P<0.05），TU200和TU300组溶菌酶（LYS）活性显著升高（P<0.05）；TU200、TU300和TU400组泥鳅肝胰腺中AST、ALT与ALP含量显著下降（P<0.05）；TU200、TU300和TU400组泥鳅肝胰腺中il-10与tgf-β的mRNA表达量显著上升（P<0.05）且il-1β与il-12的mRNA表达量显著下降（P<0.05），TU300和TU400组il-8与tnf-α的mRNA表达量显著下降（P<0.05）；TU200、TU300和TU400组泥鳅肝胰腺中fxr、bsep与shp的mRNA表达量显著升高（P<0.05），cyp7a1、cyp27a1与cyp8b1的mRNA表达量显著下降（P<0.05）。综上所述，在饲料中添加适宜TUDCA能够提高泥鳅生长性能、增强免疫功能、保护肝胰腺健康并调节胆汁酸代谢，在本试验条件下，经回归分析得出，当增重率最大时，泥鳅饲料中TUDCA的最适添加量为327.4 mg/kg。
Abstract：This study aims to investigate the effects of taurousodeoxycholic acid (TUDCA) in feed on the growth performance， immune function， hepatopancreas health， and bile acid metabolism of the loach (Misgurnus anguillicaudatus). In this experiment， TUDCA was added to the basal diet at concentrations of 0， 100， 200， 300， and 400 mg/kg， corresponding to the control (CON) group， TU100， TU200， TU300， and TU400 groups， respectively for a period of 56 days. Growth performance indicators were measured， and the expression of immune function indicators， immune function-related genes， and bile acid metabolism-related genes was detected. The results showed that， compared with the CON group， the final body weight of all experimental groups was significantly increased (P<0.05). The weight gain rate (WG) and specific growth rate (SGR) of the TU200， TU300， and TU400 groups were significantly increased (P<0.05). The protein efficiency ratio (PER) and feed conversion ratio (FCR) of the TU300 group were significantly improved (P<0.05). The serum complement 3 (C3)， complement 4 (C4)， and immunoglobulin M (IgM) contents of the TU200， TU300， and TU400 groups increased significantly (P<0.05)， and the lysozyme (LYS) activity of the TU200 and TU300 groups increased significantly (P<0.05). The AST， ALT， and ALP levels in the hepatopancreas s of loaches in the TU200， TU300， and TU400 groups decreased significantly (P<0.05). The mRNA expression of il-10 and tgf-β in the loach hepatopancreas of the TU200， TU300， and TU400 groups significantly increased (P<0.05)， and the mRNA expression of il-1β and il-12 significantly decreased (P<0.05). The mRNA expression of il-8 and tnf-α decreased significantly in the TU300 and TU400 groups (P<0.05); The mRNA expression of fxr， bsep， and shp increased significantly in the TU200， TU300， and TU400 groups (P<0.05)， while the mRNA expression of cyp7a1， cyp27a1， and cyp8b1 mRNA expression levels decreased significantly (P<0.05). In summary， TUDCA regulates growth performance， enhances immune function， and regulates hepatopancreas health and bile acid metabolism in loaches. Under the experimental conditions， a quadratic regression analysis showed that the optimal addition of TUDCA to mudfish feed was 327.4 mg/kg when the weight gain rate was highest.
[1]凌先. 泥鳅的食用价值与药用价值[J]. 技术与市场， 2009， 16(05): 116.
[2]刘敏， 王卓铎， 陈路， 等. 泥鳅养殖产业发展现状及建议[J]. 江西水产科技， 2024， (01): 44-47.
[3]蓝慧臣. 泥鳅池塘养殖技术总结[J]. 河南水产， 2023， (06): 13-14.
[4]Zhou J， Chen H， Ji H， et al. Effect of dietary bile acids on growth， body composition， lipid metabolism and microbiota in grass carp (Ctenopharyngodon idella)[J]. Aquaculture Nutrition， 2018， 24(02): 802-813.
[5]Yao T， Gu X， Liang X， et al. Tolerance assessment of dietary bile acids in common carp (Cyprinus carpio L.)fed a high plant protein diet. Aquaculture. 2021， 543: 737012.
[6]Kong B， Wang L， Chang J， et al. Mechanism of tissuespecific farnesoid X receptor in suppressing the expression of genes in bile-acid synthesis in mice[J]. Hepatology， 2012， 56(03): 1034-1043.
[7]牙程玉， 张荣臻， 王秀峰， 等. 胆汁酸代谢及肠道微生态对慢性肝脏疾病的影响[J]. 中西医结合肝病杂志， 2024， 34 (04): 366-369.
[8]徐懂， 尹美君， 王钰铭， 等. FXR-CYP7A1轴在胆汁淤积性肝病中的调控研究进展[J]. 中西医结合肝病杂志， 2021， 31 (05): 470-473.
[9]帕成周， 邬善敏. 肝细胞癌组织中CYP27A1的表达及其临床意义[J]. 中国现代普通外科进展. 2020， 23 (04): 272-276.
[10]Pan X， Zhao L， Li L， et al. Efficacy and safety of tauroursodeoxycholic acid in the treatment of liver cirrhosis: a double-blind randomized controlled trial[J]. Journal of Huazhong University of Science and Technology [Medical Sciences]， 2013， 33: 189-194.
[11]Xu X， Wang M， Li J， et al. Tauroursodeoxycholic acid alleviates hepatic ischemia reperfusion injury by suppressing the function of Kupffer cells in mice[J]. Biomed Pharmacother， 2018， 106: 1271-1281.
[12]陈娟， 冯冬婵， 麦丽珍. 牛磺熊去氧胆酸联合多烯磷酯酰胆碱治疗对非酒精性脂肪性肝病患者肝功能及血清RBP4水平的影响[J]. 川北医学院学报， 2023， 38 (11): 1517-1521.
[13]Liu Y， Mao X， Song K， et al. Dietary tauroursodeoxycholic acid supports growth and gut health in spotted seabass (Lateolabrax maculatus) under thermal stress conditions[J]. Fish & Shellfish Immunology， 2025: 110423.
[14]Liu H， Zhang X， Li K， et al. Effects of dietary of bile acid on growth performance， antioxidant capacity， liver and intestinal health and immune function in loach (Misgurnus anguillicaudatus)[J]. Aquaculture Reports， 2024， 39: 102455.
[15]Zangerolamo L， Vettorazzi J F， Solon C， et al. The bile acid TUDCA improves glucose metabolism in streptozotocin-induced Alzheimer's disease mice model[J]. Molecular and cellular endocrinology， 2021， 521: 111116.
[16]Zheng H， Xu Y C， Zhao T， et al. Dietary chenodeoxycholic acid attenuates high-fat diet-induced growth retardation， lipid accumulation and bile acid metabolism disorder in the liver of yellow catfish Pelteobagrus fulvidraco[J]. British Journal of Nutrition， 2024， 131(06): 921-934.
[17]Song M， Zhang F， Fu Y， et al. Tauroursodeoxycholic acid (TUDCA) improves intestinal barrier function associated with TGR5-MLCK pathway and the alteration of serum metabolites and gut bacteria in weaned piglets[J]. Journal of Animal Science and Biotechnology， 2022， 13(1): 73.
[18]刘思雨. 鱼类肝脏组织形态的影响因素探讨[J]. 南方农业， 2022， 16 (14): 175-177.
[19]昝丽娜， 孙峰， 张永娟， 等. miR-375、miR-146及miR-2在慢性乙型病毒性肝炎、肝硬化及肝癌患者中表达及其对肝癌的诊断意义[J]. 中国老年学杂志， 2020， 40(14): 2972-2975.
[20]姜礼燔. 鱼类肝脏疾病及防治研究[J]. 内陆水产， 2003， (01):43-44.
[21]侯静宜， 许耀升， 方揽月， 等. 左旋肉碱对鳜生长、抗氧化能力及肠道和肝脏功能的改善作用研究[J]. 动物营养学报， 2024， 36(03): 1834-1845.
[22]杨仕坤， 连俊波， 霍双杰， 等. 牛磺脱氧胆酸引起内质网应激降低和减轻T2DM肝损伤[J]. 中国组织化学与细胞化学杂志， 2022， 31(02): 125-132.
[23]Zhang W， Chen L， Feng H， et al. Rifampicin-induced injury in HepG2 cells is alleviated by TUDCA via increasing bile acid transporters expression and enhancing the Nrf2-mediated adaptive response[J]. Free Radical Biology and Medicine， 2017， 112: 24-35.
[24]Jiang J， Lu X， Dong L， et al. Enhancing growth， liver health， and bile acid metabolism of tilapia (Oreochromis niloticus) through combined cholesterol and bile acid supplementation in plant-based diets[J]. Animal Nutrition， 2024， 17: 335-346.
[25]Carroll M C. The complement system in regulation of adaptive immunity[J]. Nature immunology， 2004， 5(10): 981-986.
[26]Song Q， Xiao Y， Xiao Z， et al. Lysozymes in fish[J]. Journal of Agricultural and Food Chemistry， 2021， 69(50): 15039-15051.
[27]Jones K， Savulescu A F， Brombacher F， et al. Immunoglobulin M in health and diseases: how far have we come and what next [J]. Frontiers in Immunology， 2020， 11: 595535.
[28]Yoshikawa M， Tsujii T， Matsumura K， et al. Immunomodulatory effects of ursodeoxycholic acid on immune responses[J]. Hepatology， 1992， 16(02): 358-364.
[29]Tran H B， Chen S C， Chaung H C， et al. Molecular cloning of IL-6， IL-10， IL-11， IFN-? and modulation of pro-and anti-inflammatory cytokines in cobia (Rachycentron canadum) after Photobacterium damselae subsp. piscicida infection[J]. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology， 2019， 230: 10-18.
[30]Letterio J J， Roberts A B. Regulation of immune responses by TGF-β[J]. Annual review of immunology， 1998， 16(01): 137-161.
[31]Chen W J. TGF-β Regulation of T Cells[J]. Annual review of immunology， 2023， 41(01): 483-512.
[32]Romero-Ramírez L， Nieto-Sampedro M， Yanguas-Casás N. Tauroursodeoxycholic acid: more than just a neuroprotective bile conjugate[J]. Neural regeneration research， 2017， 12(01): 62-63.
[33]Li Q Y， Xu H Y， Yang H J. Effect of proinflammatory factors TNF-α， IL-1β， IL-6 on neuropathic pain[J]. Zhongguo Zhong yao za zhi= Zhongguo zhongyao zazhi= China journal of Chinese materia medica， 2017， 42(19): 3709-3712.
[34]祁兴震， 路侹， 谢兰， 等. 胆汁酸对动物糖脂代谢的调控及在动物生产中的应用[J]. 饲料研究， 2024， 47(04): 148-153.
[35]Xu J， Xie S， Chi S， et al. Protective effects of taurocholic acid on excessive hepatic lipid accumulation via regulation of bile acid metabolism in grouper[J]. Food & Function， 2022， 13(05): 3050-3062.
[36]冯红， 王翔. 胆盐输出泵与胆汁淤积关系的研究进展[J]. 临床合理用药杂志， 2021， 14(24): 179-181.
[37]夏斐斐， 宋冰雪， 宋雨晴， 等. 高剂量阿托伐他汀对肝损伤小鼠胆汁酸代谢的影响及其机制[J]. 精准医学杂志， 2023， 38 (05): 383-386.
基本信息:
中图分类号:S966.4
引用信息:
[1]张欣羽,孙丽,郭贵良,等.牛磺脱氧胆酸对泥鳅生长性能、免疫功能与胆汁酸代谢的影响[J].经济动物学报().
基金信息:
吉林省科技发展计划重点研发项目（20230202067NC）
2025-11-27
2025-11-27
2025-11-27