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东北林蛙（Rana dybowskii）是我国重要的经济蛙类，其蝌蚪阶段的人工养殖与管理水平直接影响后续的林蛙产量与养殖效益。光色作为一种环境因子，在动物的生长发育过程中发挥重要调节作用。本研究通过结合行为学、分子生物学与组织学手段，探讨了不同光色（黑暗、白光、红、黄、蓝、绿）对东北林蛙蝌蚪光色趋向性、HSP70家族基因表达及肠道组织结构的影响。试验以东北林蛙蝌蚪为对象，开展了为期30天的养殖研究（设5个处理组，每组3重复，12L:12D光周期）和光色选择行为观测。养殖结束后取样进行热休克蛋白相关基因的qPCR分析及肠道组织切片观察。结果表明，东北林蛙蝌蚪对蓝色光表现出明显的趋光性，而对红色光表现出回避行为。不同光色的环境显著影响了热休克蛋白（HSPs）基因的表达，蓝色光下HSP70基因表达量较低。组织学分析显示，红色光下的肠道肌层厚度显著增加，与此同时，红色光下的肠道绒毛高度显著降低，这表明不同光波长对肠道结构有特异性重塑效应。综上所述，光色不仅影响东北林蛙蝌蚪的行为和热休克蛋白基因的表达，还能够特异性地调节肠道结构和生理功能，这为优化东北林蛙人工养殖中的光环境提供了重要的科学试验依据。
Abstract：The Dybowski's frog (Rana dybowskii) is an economically important amphibian species in China. The success of tadpole rearing determines the yield and profitability of adult frogs. Spectrum， an environmental factor， play an important role in the growth and development of many animals. This study， by integrating behavioral， molecular biological and histological approaches， explored the effects of different light colors (dark， white， red， yellow， blue， green and natural light) on the light color orientation， HSP70 family gene expression and intestinal tissue structure of the tadpoles of Rana dybowskii. The experiment took the tadpoles of Rana dybowskii as the subjects and carried out a 30-day breeding study (with 5 treatment groups， 3 replicates in each group， 12L:12D photoperiod) and light color selection behavior observation. After the breeding was completed， samples were taken for qPCR analysis of heat shock protein-related genes and intestinal tissue section observation.The results show that the tadpoles of Rana dybowskii exhibit obvious phototaxis to blue light， while showing avoidance behavior towards red light. The expression of heat shock protein (HSPs) genes was significantly affected by environments with different light colors. The expression level of the HSP70 gene was relatively low under blue light. Histological analysis revealed that the thickness of the intestinal myometrium significantly increased under red light， while the height of intestinal villi significantly decreased under red light. This indicates that different light wavelengths have specific remodeling effects on intestinal structure. In conclusion， light color not only affects the behavior of the tadpoles of Rana dybowskii and the expression of heat shock protein genes， but also specifically regulates the intestinal structure and physiological functions， which provides important scientific experimental basis for optimizing the light environment in the artificial breeding of Rana dybowskii.
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基本信息:
中图分类号:S917.4
引用信息:
[1]纪浩宇,胡男,单宝龙,等.不同光色对东北林蛙蝌蚪行为、应激反应及肠道结构的影响[J].经济动物学报().
基金信息:
辽宁省农业农村厅东北林蛙专项（20250257）
2026-03-16
2026-03-16
2026-03-16