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本研究旨在探究乳酸乳球菌L22（Lactococcus lactis L22，L.lactis L22）对泥鳅（Misgurnus anguillicaudatus）生长性能、免疫功能及抗氧化能力的影响。在基础饲料中分别添加0（对照组）、1.0×10?、1.0×10?、1.0×10?和1.0×10? CFU/g的L.lactis L22，饲喂360尾初始体质量为（3.53±0.02）g的泥鳅8 w，测定其生长指标、体成分、血清生化参数、免疫指标、抗氧化酶活性及相关基因表达。结果表明：1.0×10?和1.0×10? CFU/g添加组的终末体重、增重率、特定生长率、蛋白质效率和饲料效率均显著高于对照组（P<0.05）；1.0×10? CFU/g组全鱼粗蛋白和粗脂肪含量显著提高（P<0.05）；血清AST、ALT、TBA、TG、CHOL和GLU含量在10?～10?组显著降低，而HDL-C显著上升（P<0.05）；1.0×10? CFU/g组血清补体C3、溶菌酶（LYS）和免疫球蛋白M（IgM）水平显著升高（P<0.05）；肝脏与肠道中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）和谷胱甘肽-S-转移酶（GST）活性显著增强，丙二醛（MDA）含量显著下降（P<0.05）；同时，nrf2、ho-1、nqo1和gsh-px基因表达在1.0×10? CFU/g组显著上调（P<0.05）。综上，饲料中添加1.0×10? CFU/g的L.lactis L22可显著促进泥鳅生长、增强免疫力并提高抗氧化能力。
Abstract：This study aimed to investigate the effects of Lactococcus lactis L22 (L. lactis L22) on the growth performance， immune function， and antioxidant capacity of loach (Misgurnus anguillicaudatus). A total of 360 loaches with an initial body weight of (3.53±0.02) g were fed for 8 w with basal diets supplemented with L. lactis L22 at concentrations of 0 (control group)， 1.0×10?， 1.0×10?， 1.0×10?， and 1.0×10? CFU/g， respectively. Growth indicators， body composition， serum biochemical parameters， immune indices， antioxidant enzyme activities， and related gene expression were measured. The results showed that the final body weight， weight gain rate， specific growth rate， protein efficiency， and feed efficiency in the 1.0×10? and 1.0×10? CFU/g supplementation groups were significantly higher than those in the control group (P<0.05). The crude protein and crude lipid contents in the whole fish were significantly increased in the 1.0×10? CFU/g group (P<0.05). Serum levels of AST， ALT， TBA， TG， CHOL， and GLU were significantly reduced in the 1.0×10?-1.0×10? CFU/g groups， while HDL-C was significantly increased (P<0.05). In the 1.0×10? CFU/g group， serum levels of complement C3， lysozyme (LYS)， and immunoglobulin M (IgM) were significantly elevated (P<0.05). The activities of superoxide dismutase (SOD)， catalase (CAT)， glutathione peroxidase (GSH-Px)， and glutathione-S-transferase (GST) in the liver and intestine were significantly enhanced， while malondialdehyde (MDA) content was significantly reduced (P<0.05). Meanwhile， the expression of nrf2， ho-1， nqo1， and gsh-px genes was significantly up-regulated in the 1.0×10? CFU/g group (P<0.05). In conclusion， dietary supplementation with L. lactis L22 at 1.0×10? CFU/g significantly promotes growth， enhances immunity， and improves antioxidant capacity in loach.
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基本信息:
中图分类号:S966.4
引用信息:
[1]方琼亚,杨炳坤,刘铁钢,等.乳酸乳球菌L22对泥鳅生长、免疫功能及抗氧化能力的影响[J].经济动物学报().
基金信息:
吉林省科技发展计划青年成长科技项目（20240602051RC）
2025-12-10
2025-12-10
2025-12-10