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以中畜长白半番鸭为试验材料，通过胚蛋注射技术探究L-组氨酸对鸭胚肝脏脂肪酸代谢的影响。在17胚龄（E17）向卵黄囊内注射不同剂量的L-组氨酸（0、0.35、0.7和1.4 μg/egg），E21时测定胚胎和肝脏重量，并采集肝脏样品，检测脂滴面积、脂肪酸代谢相关基因和蛋白表达。组织学染色观察发现，L-组氨酸处理增加了肝脏脂滴数量和面积。RT-qPCR结果显示，0.35 μg/egg L-组氨酸注射组中脂肪酸氧化相关基因AMPKα1、CPT1A、PPARα的mRNA表达水平显著升高（P ＜0.05），而1.4 μg/egg L-组氨酸注射组中脂肪酸合成相关基因FASN、ACC、SREBP1、PPARγ、SCD基因的mRNA表达水平显著升高（P ＜0.05）。Western blot结果显示，0.35 μg/egg L-组氨酸注射组中AMPKα1、PPARα蛋白的表达水平显著上调（P ＜0.05），1.4 μg/egg L-组氨酸注射组中FASN、ACC和SREBP1蛋白的表达水平显著升高（P ＜0.05）。综上，L-组氨酸处理通过调控脂质合成和氧化相关基因的mRNA或蛋白的表达，促进鸭胚肝脏脂肪沉积，增加肝脏重量。
Abstract：Zhongxu Changbai Mule Ducks were used as experimental materials to investigate the effect ofL-Histidine(L-His) on hepatic fatty acid metabolism in duck embryos via in ovo injection technology. Different doses of L-His were injected into eggs at embryonic age 17 (E17). At E21， embryo and liver weights were measured， and liver samples were collected to determine lipid droplet area and the expression levels of genes and proteins related to fatty acid metabolism. Histological staining showed that L-His treatment increased the number and area of lipid droplets in the liver. RT-qPCR results reverled that the mRNA expression levels of fatty acid oxidation-related genes AMPKα1， CPT1A and PPARα were significantly up-regulated in the 0.35 μg/egg L-His group (P < 0.05)， while the mRNA expression levels of fatty acid synthesis-related genes FASN， ACC， SREBP1， PPARγ and SCD were significantly increased in the 1.4 μg/egg L-His group (P < 0.05). Western blot analysis demonstrated that the protein expression levels of AMPKα1 and PPARα were significantly up-regulated in the 0.35 μg/egg L-His group (P < 0.05)， whereas those of FASN， ACC， and SREBP1 were significantly elevated in the 1.4 μg/egg L-His group (P < 0.05). In conclusion， L-His facilitates hepatic lipid deposition via regulating mRNA and protein expressions of fatty acid synthesis and oxidation-related genes， thus increasing liver weight..
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基本信息:
中图分类号:S834
引用信息:
[1]薛贵珍,李新月,周宇轩,等.胚蛋注射L-组氨酸对鸭胚肝脏脂肪酸代谢的影响[J].经济动物学报().
基金信息:
吉林省畜牧局-吉林省特色水禽高产群体繁育关键技术研究与推广项目（2024）; 吉林省科技厅-吉林省企业“科技创新专家”项目（2025）;吉林省科技厅-吉林省科学家工作室项目（2025）
2026-03-27
2026-03-27
2026-03-27