| 207 | 0 | 14 |
| 下载次数 | 被引频次 | 阅读次数 |
水貂阿留申病（AD）是一种由水貂阿留申病毒（AMDV）引起的自身免疫性疾病，严重影响水貂的免疫系统。采用蛋白质组学方法，分析了AMDV-DL125强毒株感染F81细胞后的差异表达蛋白质，并通过质谱技术进行鉴定。结果表明：共筛选出579个差异蛋白，其中有283个上调，296个下调；GO注释分析结果表明，差异表达蛋白显著富集于多个生物学过程，包括分子功能、生物过程和细胞组成等；KEGG富集分析结果表明，差异表达蛋白主要富集在PI3K-AKT信号通路和MAPK信号通路等。用RT-qPCR方法对蛋白质组测序结果进行验证，编码差异蛋白质的BCL-XL,FOSL1,JNK,SQLE和FDFT1基因的mRNA表达趋势与测序结果一致。试验通过探究AMDV-DL125强毒株感染F81细胞后蛋白表达水平，鉴定了多个差异表达蛋白质，研究结果为进一步揭示AMDV与宿主的互作关系及其致病机制提供依据。
Abstract：Mink Aleutian disease(AD) is an autoimmune disease caused by mink Aleutian virus(AMDV) that severely affects the immune system of minks. In this study, proteomic methods were used to analyze the differentially expressed proteins of F81 cells infected by AMDV-DL125 strain. Five hundred and seventy-nine differentially expressed proteins were identified by mass spectrometry, of which 283 were up-regulated and 296 were down-regulated. GO annotation analysis showed that differentially expressed proteins were significantly enriched in multiple biological processes, including molecular function, biological process and cellular component. The results of KEGG enrichment analysis showed that the differentially expressed proteins were mainly concentrated in the PI3K-AKT and MAPK signaling pathways. The results of proteomic sequencing were verified by RT-qPCR. The results showed that the mRNA expression trends of 5 different genes including BCL-XL, FOSL1, JNK, SQLE and FDFT1 were consistent with the sequencing results. In this study, the changes in protein expression levels after infection of F81 cells by AMDV-DL125 strain were studied, and a variety of differentially expressed proteins were identified, which provided a basis for further revealing the relationship between AMDV and host and the pathogenesis.
[1]Bloom M E, Kanno H, Mori S, et al. Aleutian mink disease:Puzzles and paradigms[J]. Infectious Agents and Disease,1994, 3(6):279-301.
[2]Broll S, Alexandersen S. Investigation of the pathogenesis of transplacental transmission of Aleutian mink disease parvovirus in experimentally infected mink[J]. Journal of Virology,1996, 70(3):1455-1466.
[3]Mañas S, Ceña J C, Ruiz-Olmo J, et al. Aleutian mink disease parvovirus in wild riparian carnivores in Spain[J]. Journal of Wildlife Diseases, 2001, 37(1):138-144.
[4]van Dawen S, Kaaden O R, Roth S. Propagation of Aleutian disease parvovirus in cell line CCC clone 81[J]. Archives of Virology, 1983, 77(1):39-50.
[5]逄锦颖.山东省水貂胴体内ADV、MEV和CDV检测与分离株的变异及ADV对小鼠安全性分析[D].泰安：山东农业大学，2018.
[6]Dworak L J, Wolfinbarger J B, Bloom M E. Aleutian mink disease parvovirus infection of K562 cells is antibody-dependent and is mediated via an Fc(gamma)RⅡreceptor[J]. Archives of Virology, 1997, 142(2):363-373.
[7]Knuuttila A, Aaltonen K, Virtala A K, et al. Aleutian mink disease virus in free-ranging mustelids in Finland-a cross-sectional epidemiological and phylogenetic study[J]. The Journal of General Virology, 2015, 96(6):1423-1435.
[8]Farid A H. Aleutian mink disease virus in furbearing mammals in Nova Scotia, Canada[J]. Acta Veterinaria Scandinavica, 2013, 55(1):10.
[9]Canuti M, McDonald E, Graham S M, et al. Multi-host dispersal of known and novel carnivore amdoparvoviruses[J]. Virus Evolution, 2020, 6(2):veaa072.
[10]Best S M, Wolfinbarger J B, Bloom M E. Caspase activation is required for permissive replication of aleutian mink disease parvovirus in vitro[J]. Virology, 2002, 292(2):224-234.
[11]Knuuttila A, Uzcátegui N, Kankkonen J, et al. Molecular epidemiology of Aleutian mink disease virus in Finland[J].Veterinary Microbiology, 2009, 133(3):229-238.
[12]Jepsen J R, D'Amore F, Baandrup U, et al. Aleutian mink disease virus and humans[J]. Emerging Infectious Diseases,2009, 15(12):2040-2042.
[13]盛陈艳. MiCV和AMDV双重荧光定量PCR检测试剂盒的初步研制及其流行病学调查研究[D].长春：吉林农业大学，2022.
[14]Leng X, Liu D X, Li J M, et al. Genetic diversity and phylogenetic analysis of Aleutian mink disease virus isolates in North-east China[J]. Archives of Virology, 2018, 163(5):1241-1251.
[15]张定秀，井长华，王若楠，等.山东省部分地区水貂阿留申病PCR检测及病原的分离鉴定[J].经济动物学报，2025, 29(4):299-304.
[16]汪婷婷. PI3K/Akt信号通路在AMDV诱导CrFK细胞凋亡中的作用机制研究[D].长春：吉林农业大学，2023.
[17]Zou W, Wang Z K, Xiong M, et al. Human parvovirus B19utilizes cellular DNA replication machinery for viral DNA replication[J]. Journal of Virology, 2018, 92(5):e01881-17.
[18]葛桂阳，栾美慧，宫庆龙，等.水貂阿留申病毒诱导CrFK细胞凋亡信号通路的研究[J].中国预防兽医学报，2021,43(10):1089-1095.
基本信息:
DOI：10.13326/j.jea.2026.2085
中图分类号:S852.65
引用信息:
[1]宋宇,刘莹玉,梁健,等.AMDV感染F81细胞的蛋白质组学分析及部分差异蛋白验证[J].经济动物学报,2026,30(02):150-156.DOI:10.13326/j.jea.2026.2085.
基金信息:
吉林省科技发展计划项目（20220101332JC,20250203111SF）
2025-10-29
2025-10-29
2025-10-29