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四个半LIM域蛋白3（FHL3）可以通过LIM结构域与多种蛋白质相互作用，从而调控成肌细胞分化与肌细胞的形成。本试验以雌、雄梅花鹿（Cervus nippon）背最长肌、股四头肌、颈斜方肌、肱二头肌、臀大肌为样品，成功克隆出FHL3基因CDS区，长度为843bp，编码280个氨基酸；Blastp结果显示梅花鹿FHL3基因序列与马鹿相似性最高，与小鼠亲缘关系较远；生物信息学预测显示FHL3蛋白为亲水蛋白，定位于细胞核内，二级结构主要由无规则卷曲、α-螺旋和β-折叠构成；荧光定量PCR结果显示FHL3基因在肱二头肌中相对表达量最高，雌、雄间仅颈斜方肌与臀大肌存在差异表达。本试验旨在深入了解梅花鹿FHL3基因基本信息，为进一步揭示FHL3基因调控梅花鹿骨骼肌纤维类型分化机制研究奠定基础。
Abstract：Four and a half LIM domains protein 3 (FHL3) can interact with various proteins through the LIM structure domain， thereby regulating the differentiation and formation of muscle cells. This experiment took the longest back muscle， quadriceps， sternocleidomastoid， biceps， and gluteus maximus of male and female sika deer (Cervus nippon) as samples， and successfully cloned the FHL3 gene CDS region， which was 843bp in length and encoded 280 amino acids. The Blastp results showed that the FHL3 gene sequence of sika deer had the highest similarity with that of deer， and a more distant relationship with mice. Bioinformatics prediction showed that the FHL3 protein was a hydrophilic protein located in the nucleus， and its secondary structure was mainly composed of irregular curls， α-helices， and β-folds. The fluorescence quantitative PCR results showed that the FHL3 gene was highly expressed in the biceps， and there was a difference in expression between males and females only in the sternocleidomastoid and gluteus maximus. This experiment aimed to deeply understand the basic information of the FHL3 gene in sika deer， and laid the foundation for further revealing the mechanism of FHL3 gene regulating the differentiation of skeletal muscle fiber types in sika deer.
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基本信息:
中图分类号:S825
引用信息:
[1]朱涛,李和平,马瑜锴,等.梅花鹿FHL3基因克隆、生物信息学分析及组织表达[J].经济动物学报().
基金信息:
黑龙江省高校学科协同创新成果建设项目（LJGXCG2022-008）
2025-11-27
2025-11-27
2025-11-27