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为探究植物乳杆菌胞外囊泡对结肠炎的防治效果，本实验以从猪源植物乳杆菌LP中提取的胞外囊泡（LpEVs）作为研究对象，将其以不同浓度灌胃给C57BL/6小鼠后，再口服DSS诱导构建结肠炎模型。通过监测小鼠的体重变化率、疾病活动指数、结肠长度、脾脏指数，HE染色观察结肠组织病理变化，并通过ELISA检测血清中髓过氧化物酶的活性，IL-6、IL-1β和TNF-α细胞因子的表达。结果显示，DSS诱导的结肠炎小鼠体重下降，DAI指数升高，结肠缩短，脾脏指数升高，结肠组织损伤严重，血清髓过氧化物酶活性升高，促炎细胞因子IL-6、IL-1β和TNF-α表达水平升高，而在经过植物乳杆菌胞外囊泡干预后，上述结果均有所逆转，表明植物乳杆菌胞外囊泡对结肠炎具有较好的缓解作用，为揭示益生菌缓解结肠炎的机制提供理论基础。
Abstract：In order to explore the prevention and treatment effect of extracellular vesicles of Lactobacillus plantarum on colitis， extracellular vesicles (LpEVs) extracted from LP of porcine source were administered to C57BL/6 mice at different concentrations， and then DSS was used to induce colitis model.The body weight change rate， disease activity index， colon length and spleen index of mice were monitored. The pathological changes of colon tissue were observed by HE staining. The activity of myeloperoxidase in serum and the expression of IL-6， IL-1β and TNF-α cytokines were detected by ELISA.The results showed that the body weight of DSS-induced colitis mice decreased， the DAI index increased， the colon shortened， the spleen index increased， the colon tissue was seriously damaged， the serum myeloperoxidase activity increased， and the expression levels of pro-inflammatory cytokines IL-6， IL-1β and TNF-α increased. After the intervention of Lactobacillus plantarum extracellular vesicles， the above results were reversed.It shows that Lactobacillus plantarum extracellular vesicles have a good mitigation effect on colitis， which provides a theoretical basis for revealing the mechanism of probiotics to alleviate colitis.
[1] Isabelle Cleynen，Gabrielle Boucher，Luke Jostins， et al. Inherited determinants of Crohn's disease and ulcerative colitis phenotypes: a genetic association study. The Lancet. 2016;387 (10014):156-167.
[2] Kaplan GG. The global burden of IBD: from 2015 to 2025. Nat Rev Gastroenterol Hepatol. 2015;12(12):720-727.
[3] Sirine Imad Andari，Hadi Hussein，Sukayna Fadlallah， et al. Epstein–Barr Virus DNA Exacerbates Colitis Symptoms in a Mouse Model of Inflammatory Bowel Disease. Viruses. 2021;13 (7):1272-1272.
[4] Raja Atreya，Jonas Mudter，Susetta Finotto， et al. Blockade of interleukin 6 trans signaling suppresses T-cell resistance against apoptosis in chronic intestinal inflammation: Evidence in Crohn disease and experimental colitis in vivo. Nature Medicine. 2000;6 (5):583-588.
[5] Yasuyuki Kai，Ichiro Takahashi，Hiromichi Ishikawa， et al. Colitis in mice lacking the common cytokine receptor γ chain is mediated by IL-6-producing CD4+ T cells. Gastroenterology. 2005;128 (4):922-934.
[6] Nobuhiko Kamada，Tadakazu Hisamatsu，Susumu Okamoto， et al. Unique CD14+ intestinal macrophages contribute to the pathogenesis of Crohn disease via IL-23/IFN-γ axis. Journal of Clinical Investigation. 2008;0 (0):0-0.
[7] Warren Strober，Ivan J. Fuss，Richard S. Blumberg. The Immunology of Mucosal Models of Inflammation. Annual Review of Immunology. 2002;20 (1):495-549.
[8] Okayasu I， Hatakeyama S， Yamada M， Ohkusa T， Inagaki Y， Nakaya R. A novel method in the induction of reliable experimental acute and chronic ulcerative colitis in mice. Gastroenterology. 1990;98(3):694-702.
[9] Brunette Katsandegwaza，William Horsnell，Katherine A. Smith. Inflammatory Bowel Disease: A Review of Pre-Clinical Murine Models of Human Disease. International Journal of Molecular Sciences. 2022;23 (16):9344-9344.
[10] Bo Shen，Jean Paul Achkar，Bret A. Lashner， et al. A Randomized Clinical Trial of Ciprofloxacin and Metronidazole to Treat Acute Pouchitis. Inflammatory Bowel Diseases. 2001;7 (4):301-305.
[11] Mengmeng Niu，Huan-Xin Guo，Jun-Wu Cai， et al. Bifidobacterium breve Alleviates DSS-Induced Colitis in Mice by Maintaining the Mucosal and Epithelial Barriers and Modulating Gut Microbes. Nutrients. 2022;14 (18):3671-3671.
[12] Yanni Pan，Yujing Ning，Jing Hu， et al. The Preventive Effect of Lactobacillus plantarum ZS62 on DSS-Induced IBD by Regulating Oxidative Stress and the Immune Response. Book Publisher International (a part of SCIENCEDOMAIN International). 2022;0 (0):18-37.
[13] Tae-Kil Eom，Yong Sung Kim，Chang Hwan Choi， et al. Current understanding of microbiota- and dietary-therapies for treating inflammatory bowel disease. Journal of Microbiology. 2018;56 (3):189-198.
[14] Peter A. Bron，Michiel Kleerebezem，Robert Jan Brummer， et al. Can probiotics modulate human disease by impacting intestinal barrier function?. British Journal of Nutrition. 2017;117 (1):93-107.
[15] 高涵，吴影，铁珊珊，等.凝结芽孢杆菌CGMCC 9951对小鼠溃疡性结肠炎的保护作用[J].食品与发酵工业，2023，49(19):90-96.
[16] Sheng-Te Wang，Yingying Wang，Jingmin Huang， et al. THZ2 Ameliorates Mouse Colitis and Colitis-Associated Colorectal Cancer. Biomedicines. 2024;12 (3):679-679.
[17] Ruoran Shi，Fazheng Yu，Xueyu Hu， et al. Protective Effect of Lactiplantibacillus plantarum subsp. plantarum SC-5 on Dextran Sulfate Sodium—Induced Colitis in Mice. Foods. 2023;12 (4):897-897.
[18] 吴昕雨.具核梭杆菌胞外囊泡感染CRC细胞的作用机制及双歧杆菌对其的生物调控[D].东北农业大学，2023.
[19] Brooke L. Deatherage，Brad T. Cookson. Membrane Vesicle Release in Bacteria， Eukaryotes， and Archaea: a Conserved yet Underappreciated Aspect of Microbial Life. Infection and Immunity. 2012;80 (6):1948-1957.
[20] Y.-S. Kim，Eun Jung Choi，Won Hee Lee， et al. Extracellular vesicles， especially derived from Gram-negative bacteria， in indoor dust induce neutrophilic pulmonary inflammation associated with both Th1 and Th17 cell responses. Clinical & Experimental Allergy. 2013;43 (4):443-454.
[21] José Alberto Molina-Tijeras，Júlio Gálvez，María Elena Rodríguez-Cabezas. The Immunomodulatory Properties of Extracellular Vesicles Derived from Probiotics: A Novel Approach for the Management of Gastrointestinal Diseases. Nutrients. 2019;11 (5):1038-1038.
[22] Min Hye Kim，Sang Joon Choi，Hyun Il Choi， et al. Lactobacillus plantarum-derived Extracellular Vesicles Protect Atopic Dermatitis Induced byStaphylococcus aureus-derived Extracellular Vesicles. Allergy， Asthma and Immunology Research. 2018;10 (5):516-516.
[23] Ruichang Gao，Yang Shen，Wanghui Shu， et al. Sturgeon hydrolysates alleviate DSS-induced colon colitis in mice by modulating NF-κB， MAPK， and microbiota composition. Food & Function. 2020;11 (8):6987-6999.
[24] Zhaobei Cai，Shu Wang，Jiannan Li. Treatment of Inflammatory Bowel Disease: A Comprehensive Review. Frontiers in Medicine. 2021;8 (0):0-0.
[25] Emily Minor，Amanda Stewart，Justin Kupec， et al. Oral iron supplementation exacerbates dextran sulfate sodium (DSS)-induced colitis in mice. Physiology. 2023;38 (S1):0-0.
[26] Hye‐Jung Moon，Youn‐Soo Cha，Kyung-Ah Kim. Blackcurrant Alleviates Dextran Sulfate Sodium (DSS)-Induced Colitis in Mice. Foods. 2023;12 (5):1073-1073.
[27] Alba Rodríguez-Nogales，Francesca Algieri，José Garrido-Mesa， et al. Intestinal anti-inflammatory effect of the probiotic Saccharomyces boulardii in DSS-induced colitis in mice: Impact on microRNAs expression and gut microbiota composition. The Journal of Nutritional Biochemistry. 2018;61 (0):129-139.
[28] Michele Biagioli，Adriana Carino，Cristina Di Giorgio， et al. Discovery of a Novel Multi-Strains Probiotic Formulation with Improved Efficacy toward Intestinal Inflammation. Nutrients. 2020;12 (7):1945-1945.
[29] 杨硕，唐宗馨，段勃帆，等.双歧杆菌及其制剂对炎症性肠病作用机制研究进展[J].食品科学，2023，44(05):275-281.
[30] 尹玲，彭辉，张铭辉，等.益生菌的肠道保健机制[J].中国当代医药，2013，20(22):15-17.
[31] 张翼，张晨虹，申剑，等.膳食诱导肥胖大鼠模型的肠道菌群结构及其特异分子标识物[J].中国微生态学杂志，2009，21(04):318-322+326.
[32] Qing Gu，Chenlan Xia，Nana Liu， et al. Lactobacillus plantarum ZJ316 alleviates ulcerative colitis by inhibiting inflammation and regulating short-chain fatty acid levels and the gut microbiota in a mouse model. Food & Function. 2023;14 (9):3982-3993.
[33] Mingming Sun，Wei Wu，Zhanju Liu， et al. Microbiota metabolite short chain fatty acids， GPCR， and inflammatory bowel diseases. Journal of Gastroenterology. 2016;52 (1):1-8.
[34] Josep Bassaganya‐Riera，Monica Viladomiu，Mireia Pedragosa， et al. Probiotic Bacteria Produce Conjugated Linoleic Acid Locally in the Gut That Targets Macrophage PPAR γ to Suppress Colitis. PLOS ONE. 2012;7 (2):e31238-e31238.
[35] Fang Yan，D. Brent Polk. Probiotics and Probiotic-Derived Functional Factors—Mechanistic Insights Into Applications for Intestinal Homeostasis. Frontiers in Immunology. 2020;11 (0):0-0.
[36] 孟令玉，石阳倩，张春亮，等.益生菌胞外囊泡与疾病的相关研究进展[J].中国微生态学杂志，2022，34(08):983-987.
[37] Lingjun Tong，Xinyi Zhang，Haining Hao， et al. Lactobacillus rhamnosus GG Derived Extracellular Vesicles Modulate Gut Microbiota and Attenuate Inflammatory in DSS-Induced Colitis Mice. Nutrients. 2021;13 (10):3319-3319.
[38] 陈瑶， 黄维莉. 中性粒细胞在UC中炎症机制的研究进展[J]. 临床医学进展， 2024， 14(6): 1485-1489.
[39] Jiang Xe，Yang Sm，Zhou Xj， et al. Effects of mesalazine combined with bifid triple viable on intestinal flora， immunoglobulin and levels of cal， MMP-9， and MPO in feces of patients with ulcerative colitis. DOAJ (DOAJ: Directory of Open Access Journals). 2020;0 (0):0-0.
[40] 朱国民，廖福全，郑伟华.溃疡性结肠炎患者血清IL-1β、C反应蛋白及髓过氧化物酶水平与肠道菌群的关系[J].中国微生态学杂志，2023，35(12):1434-1438.
基本信息:
中图分类号:R574.62;R-332
引用信息:
[1]单心,张仕涵,屈广隆,等.植物乳杆菌胞外囊泡对DSS诱导结肠炎的缓解作用[J].经济动物学报().
基金信息:
吉林省科技厅项目（20240601058RC）; 国家自然科学基金（U21A20261，32273043）; 吉林省大学生创新创业训练计划项目（S202310193135，s202410193009）
2025-04-15
2025-04-15
2025-04-15