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牛精液冷冻过程中容易受到氧化应激的影响，导致精子细胞受到不同程度的氧化损伤。纳米茶多酚具有抗氧化性，抑制自由基产生的脂质过氧化。为探究纳米茶多酚对牛精液冷冻保存效果的影响，本研究以西门塔尔种公牛精液为材料，在稀释液中添加不同浓度（0 μg/mL、2 μg/mL、4 μg/mL、6 μg/mL）的纳米茶多酚，通过冷冻保存后检测精子活力、运动学参数、顶体完整率及氧化应激指标来评定纳米茶多酚的作用。结果表明，与对照组相比，4 μg/mL纳米茶多酚可显著提高了精子活力（P<0.05），精子直线速度（VSL）、曲线速度（VCL）等运动学参数和顶体完整率显著提升（P<0.05）。此外，4 μg/mL纳米茶多酚组的过氧化氢酶（CAT）、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）活性显著升高（P<0.05），丙二醛（MDA）含量显著降低（P<0.05）。结果表明，在稀释液中添加4 μg/mL纳米茶多酚可有效减轻冷冻过程中牛精子的氧化损伤，提高冷冻后精液品质。
Abstract：During the cryopreservation of bull semen， oxidative stress could easily occur， leading to varying degrees of oxidative damage to sperm cells. Nano-tea polyphenols possess antioxidant properties that inhibit lipid peroxidation caused by free radicals. To investigate the effect of nano-tea polyphenols on the cryopreservation efficiency of bull semen， this study used Simmental bull semen as the experimental material and added different concentrations (0 μg/mL， 2 μg/mL， 4 μg/mL， 6 μg/mL) of nano-tea polyphenols to the diluent. After cryopreservation， sperm motility， kinematic parameters， acrosome integrity， and oxidative stress indicators were evaluated. The results showed that compared with the control group， the 4 μg/mL nano-tea polyphenol treatment group significantly improved sperm motility (P < 0.05)， kinematic parameters such as straight-line velocity (VSL) and curvilinear velocity (VCL) (P < 0.05)， and acrosome integrity (P < 0.05). Additionally， this group exhibited significantly increased activities of catalase (CAT)， superoxide dismutase (SOD)， and glutathione peroxidase (GSH-Px) (P < 0.05)， along with a significant reduction in malondialdehyde (MDA) content (P < 0.05).These findings indicate that adding 4 μg/mL nano-tea polyphenols to the diluent could effectively mitigate oxidative damage to sperm during cryopreservation and enhance post-thaw semen quality.
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基本信息:
中图分类号:S823
引用信息:
[1]郑秋菊,郭乐薇,王宇诺,等.纳米茶多酚对牛精液冷冻效果的影响[J].经济动物学报().
基金信息:
吉林省肉牛产业化发展重大科技专项（YDZJ202203CGZH041）; 国家自然科学基金区域创新发展联合基金项目（U22A20509）
2025-07-02
2025-07-02
2025-07-02