| 10 | 0 | 11 |
| 下载次数 | 被引频次 | 阅读次数 |
为了解黑龙江省某规模化养猪场猪繁殖与呼吸综合征病毒(PRRSV)的遗传变异特性，本研究在2021-2024年间在该猪场共采集354份样品，通过实时荧光定量PCR检测样本，结果显示有38份样本为阳性，总阳性率为10.73%，2021年至2024年阳性率无明显递增或递减趋势。分别对获得的38份阳性毒株序列进行遗传进化、同源性和编码氨基酸的变异特性分析。基于ORF5的遗传进化分析结果显示，共筛选获得3株亚谱系1.8、25株亚谱系1.5以及10株谱系8。同源性分析证明38株PRRSV毒株核苷酸序列相似性为82.7%-100%，氨基酸序列相似性为81.7%-99.5%。氨基酸突变分析表明，GP5蛋白的信号肽、中和表位、T细胞表位和B细胞表位均发生氨基酸突变，其中10株PRRSV同时保留R13和R151毒力位点。本研究通过对2021-2024年间黑龙江省某猪场PRRSV流行情况的长期监测，并对阳性样本进行分子特征分析，综合阐明该猪场PRRSV流行特点及遗传多样性，为PRRSV的流行病学状况及疫情预防和控制提供理论基础。
Abstract：To characterize the genetic variation of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) in a large-scale pig farm in Heilongjiang Province， a total of 354 samples were collected from the farm between 2021 and 2024. RT-qPCR detection revealed 38 positive samples， yielding an overall positivity rate of 10.73%. No significant upward or downward trend in annual positivity rates was observed from 2021 to 2024. Phylogenetic， homology， and amino acid variation analyses were performed on the 38 positive strains. Phylogenetic analysis based on the ORF5 gene showed that three strains were classified into sublineage 1.8， 25 into sublineage 1.5， and 10 into lineage 8. Homology analysis indicated that the nucleotide sequence identities among the 38 PRRSV strains ranged from 82.7% to 100%， and the amino acid sequence identities ranged from 81.7% to 99.5%. Amino acid mutation analysis revealed that the signal peptide， neutralizing epitope， T?cell epitope， and B?cell epitope of the GP5 protein all contained amino acid substitutions. Among these strains， 10 retained both virulence-related residues R13 and R151. Through long?term monitoring of PRRSV prevalence in a swine farm in Heilongjiang Province from 2021 to 2024， combined with molecular characterization of the positive samples， this study comprehensively elucidates the epidemic characteristics and genetic diversity of PRRSV in the farm， providing a theoretical basis for understanding the epidemiological status of PRRSV and for the prevention and control of future outbreaks.
[1] Nathues H， Alarcon P， Rushton J， Jolie R， Fiebig K， Jimenez M， Geurts V， Nathues C. Cost of porcine reproductive and respiratory syndrome virus at individual farm level - An economic disease model. Prev Vet Med. 2017 Jul 1;142:16-29.
[2] Amona FM， Pang Y， Gong X， Wang Y， Fang X， Zhang C， Chen X. Mechanism of PRRSV infection and antiviral role of polyphenols. Virulence. 2024 Dec;15(1):2417707.
[3] Franzo G， Faustini G， Legnardi M， Cecchinato M， Drigo M， Tucciarone CM. Phylodynamic and phylogeographic reconstruction of porcine reproductive and respiratory syndrome virus (PRRSV) in Europe: Patterns and determinants. Transbound Emerg Dis. 2022 Sep;69(5):e2175-e2184.
[4] Shi M， Lam TT， Hon CC， Murtaugh MP， Davies PR， Hui RK， Li J， Wong LT， Yip CW， Jiang JW， Leung FC. Phylogeny-based evolutionary， demographical， and geographical dissection of North American type 2 porcine reproductive and respiratory syndrome viruses. J Virol. 2010 Sep;84(17):8700-11.
[5] Lunney JK， Fang Y， Ladinig A， Chen N， Li Y， Rowland B， Renukaradhya GJ. Porcine Reproductive and Respiratory Syndrome Virus (PRRSV): Pathogenesis and Interaction with the Immune System. Annu Rev Anim Biosci. 2016;4:129-54.
[6] Shi M， Lam TT， Hon CC， Hui RK， Faaberg KS， Wennblom T， Murtaugh MP， Stadejek T， Leung FC. Molecular epidemiology of PRRSV: a phylogenetic perspective. Virus Res. 2010 Dec;154(1-2):7-17.
[7] Li C， Li J， Gong B， Xu H， Guo Z， Xiang L， Zhang S， Sun Q， Zhao J， Zhang M， Tang YD， Leng C， Wu J， Wang Q， Peng J， Zhou G， Liu H， An T， Cai X， Tian ZJ， Zhang H. A lineage 1 branch porcine reproductive and respiratory syndrome virus live vaccine candidate provides broad cross-protection against HP-like PRRSV in piglets. Virulence. 2025 Dec;16(1):2451754.
[8] Guo Z， Chen XX， Li X， Qiao S， Deng R， Zhang G. Prevalence and genetic characteristics of porcine reproductive and respiratory syndrome virus in central China during 2016-2017: NADC30-like PRRSVs are predominant. Microb Pathog. 2019 Oct;135:103657.
[9] Zhang H， Luo Q， Zheng Y， Sha H， Li G， Kong W， Huang L， Zhao M. Genetic Variability and Recombination of the NSP2 Gene of PRRSV-2 Strains in China from 1996 to 2021. Vet Sci. 2023 Apr 29;10(5):325.
[10] Xu H， Song S， Zhao J， Leng C， Fu J， Li C， Tang YD， Xiang L， Peng J， Wang Q， Zhao H， An T， Cai X， Zhang H， Tian ZJ. A potential endemic strain in China: NADC34-like porcine reproductive and respiratory syndrome virus. Transbound Emerg Dis. 2020 Jul;67(4):1730-1738.
[11] 高小鹏，员晓庆，谢佩琴，等.2023－2024年广东省PRRSV分子流行病学调查及ORF5基因遗传变异分析[J/OL].中国动物传染病学报，1-10.
[12] Zhou L， Han J， Yang H. The evolution and diversity of porcine reproductive and respiratory syndrome virus in China. Vet Microbiol. 2024 Nov;298:110252.
[13] Zhao K， Ye C， Chang XB， Jiang CG， Wang SJ， Cai XH， Tong GZ， Tian ZJ， Shi M， An TQ. Importation and Recombination Are Responsible for the Latest Emergence of Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus in China. J Virol. 2015 Oct;89(20):10712-6.
[14] Wu Z， Chang T， Wang D， Zhang H， Liu H， Huang X， Tian Z， Tian X， Liu D， An T， Yan Y. Genomic surveillance and evolutionary dynamics of type 2 porcine reproductive and respiratory syndrome virus in China spanning the African swine fever outbreak. Virus Evol. 2024 Feb 9;10(1):veae016.
[15] Zhang HL， Zhang WL， Xiang LR， Leng CL， Tian ZJ， Tang YD， Cai XH. Emergence of novel porcine reproductive and respiratory syndrome viruses (ORF5 RFLP 1-7-4 viruses) in China. Vet Microbiol. 2018 Aug;222:105-108.
[16] Bao H， Li X. Emergence and spread of NADC34-like PRRSV in China. Transbound Emerg Dis. 2021 Nov;68(6):3005-3008.
[17] Xu H， Li C， Li W， Zhao J， Gong B， Sun Q， Tang YD， Xiang L， Leng C， Peng J， Wang Q， Meng F， Yu Y， An T， Cai X， Tian ZJ， Zhang H. Novel characteristics of Chinese NADC34-like PRRSV during 2020-2021. Transbound Emerg Dis. 2022 Sep;69(5):e3215-e3224.
[18] Luo Q， Zheng Y， Zhang H， Yang Z， Sha H， Kong W， Zhao M， Wang N. Research Progress on Glycoprotein 5 of Porcine Reproductive and Respiratory Syndrome Virus. Animals (Basel). 2023 Feb 23;13(5):813.
[19] Wang X， Zhang K， Mo Q， Chen G， Lv J， Huang J， Pang Y， Wang H， Liu W， Huang K， Min X， Ren T， Ouyang K， Chen Y， Huang W， Wei Z. The Emergence and Pathogenesis of Recombinant Viruses Associated with NADC34-like Strains and the Predominant Circulating Strains of Porcine Reproductive and Respiratory Syndrome Virus in Southern China. Viruses. 2022 Jul 31;14(8):1695.
[20] Allende R， Kutish GF， Laegreid W， Lu Z， Lewis TL， Rock DL， Friesen J， Galeota JA， Doster AR， Osorio FA. Mutations in the genome of porcine reproductive and respiratory syndrome virus responsible for the attenuation phenotype. Arch Virol. 2000;145(6):1149-61.
[21] Ostrowski M， Galeota JA， Jar AM， Platt KB， Osorio FA， Lopez OJ. Identification of neutralizing and nonneutralizing epitopes in the porcine reproductive and respiratory syndrome virus GP5 ectodomain. J Virol. 2002 May;76(9):4241-50.
[22] Kim WI， Kim JJ， Cha SH， Wu WH， Cooper V， Evans R， Choi EJ， Yoon KJ. Significance of genetic variation of PRRSV ORF5 in virus neutralization and molecular determinants corresponding to cross neutralization among PRRS viruses. Vet Microbiol. 2013 Feb 22;162(1):10-22.
基本信息:
中图分类号:S852.651
引用信息:
[1]赵昱凯,周可鑫,仲宇思,等.黑龙江省某猪场PRRSV分子特性分析[J].经济动物学报().
基金信息:
吉林省教育厅科学研究项目（JJKH20261610KJ）
2026-05-28
2026-05-28
2026-05-28