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本研究旨在应用生物信息学方法分析蜜蜂残翅病毒的前导蛋白结构，基于六个不同寄主:西方蜜蜂（Apis mellifera）、安南竹节虫（Medauroidea extradentata）、狄斯瓦螨（Varroa destructor）以及本实验室提取的三种植物叶子花（Bougainvillea spectabilis Willd.) ，紫藤花（Wisteria sinensis.），迎春花（Jasminum nudiflorum Lindl.）的DWV毒株的全基因组序列数据，我们预测并分析了蜜蜂残翅病毒前导蛋白的理化性质、亲疏水性、跨膜区、信号肽、磷酸化和糖基化位点、二级结构和细胞表位。发现所有六种前导蛋白均含有磷酸化位点、糖基化位点和表位，但存在细微差异。这些位点可能影响蛋白活化、细胞取向模式及感染特定细胞的能力，对抗病毒抑制剂设计具有指导意义。研究结果为DWV跨物种传播研究提供了生物信息学基础。
Abstract：This study aims to analyze the structure of the Deformed Wing Virus (DWV) leader proteins using bioinformatics methods， selecting DWV full sequences extracted from six organisms， including Apis mellifera， Medauroidea extradentata，Varroa destructor， and three types of plants (Bougainvillea spectabilis Willd.， Wisteria sinensis.， and Jasminum nudiflorum Lindl.) extracted in our laboratory. Through prediction and analysis of the physicochemical properties，hydrophilicity and hydrophobicity， transmembrane regions， signal peptides， phosphorylation and glycosylation sites，secondary structures， and cellular epitopes of DWV leader proteinss， we found that all six leader proteins contain phosphorylation sites， glycosylation sites， and epitopes， but with subtle differences. These sites may influence protein activation， cellular orientation patterns， and the ability to infect specific cells， providing guidance for the design of antiviral inhibitors. The results of this study provide a bioinformatics foundation for the research on the cross-species transmission of DWV.
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基本信息:
中图分类号:S895
引用信息:
[1]龚沛勋,王斌,董坤,等.蜜蜂残翅病毒不同寄主毒株的前导蛋白生物信息学分析[J].经济动物学报().
基金信息:
云南省国际科技特派员项目（202203AK140020）; 国家现代蜂产业技术体系项目（CARS-44-kxj13）
2024-12-06
2024-12-06
2024-12-06