| 229 | 0 | 3 |
| 下载次数 | 被引频次 | 阅读次数 |
为探究水飞蓟素对黄颡鱼的生长性能、体组成及非特异性免疫指标的影响,试验配制5种等氮(43.96%)、等能(17.94 kJ/kg)且不同水飞蓟素水平(0、50、100、150和200 mg/kg)的配合饲料,每种饲料设置3个重复,每个重复饲喂黄颡鱼30尾,暂养2周后,进行为期8周的饲养试验。结果显示:S3组的FBW、WGR、SGR和PER与S4组无显著性差异(P<0.05),但均显著高于S1组、S2组和S5组(P<0.05);S3组与S4的FCR无显著性差异(P<0.05),但均显著低于S1组、S2组和S5组(P<0.05);各试验组的SR均显著高于对照组S1组(P<0.05)。各组分的粗蛋白含量无显著性差异(P>0.05);S3组的粗脂肪和水分含量与S4组和S5组无显著性差异(P>0.05),但显著低于对照组S1组(P>0.05);S3组的粗灰分含量显著高于S2组和S1组(P<0.05),显著低于S4组和S5组(P<0.05)。水飞蓟素对各组的AKP活性无显著性影响(P>0.05);S3组的SOD、LZM和ACP活性与S4组无显著性差异(P>0.05),但显著高于S1组、S2组和S5组(P<0.05)。在本试验条件下,饲料中添加水飞蓟素能够提高鱼体的生长性能,降低鱼体粗脂肪含量,提高鱼体非特异性免疫力,且最适添加量为100mg/kg时效果最佳。
Abstract:To investigate the effects of silymarin on growth performance, body composition, and non-specific immune indices of Pelteobagrus fulvidraco, five isonitrogenous (43.96%) and isoenergetic (17.94 kJ/kg) diets with varying silymarin levels (0, 50, 100, 150, and 200 mg/kg) were formulated. Each diet was assigned to three replicates, with 30 Pelteobagrus fulvidraco per replicate. After a 2-week acclimation, an 8-week feeding trial was conducted. Results showed that the S3 group (100 mg/kg) exhibited no significant differences in final body weight (FBW), weight gain rate (WGR), specific growth rate (SGR), or protein efficiency ratio (PER) compared to the S4 group (150 mg/kg) (P > 0.05), but these parameters were significantly higher than those of the S1 (control), S2 (50 mg/kg), and S5 (200 mg/kg) groups (P < 0.05). The feed conversion ratio (FCR) of S3 and S4 groups showed no significant difference (P > 0.05) but was significantly lower than that of S1, S2, and S5 groups (P < 0.05). Survival rate (SR) in all silymarin-supplemented groups was significantly higher than in the control group (P < 0.05). crude protein (CP) content showed no significant differences among groups (P > 0.05). ether extract, crude fat (EE) and moisture contents in S3, S4, and S5 groups were significantly lower than in S1 (P < 0.05), while crude ash (Ash) content in S3 was significantly higher than in S1 and S2 (P < 0.05) but lower than in S4 and S5 (P < 0.05). Silymarin had no significant effect on alkaline phosphatase (AKP) activity (P > 0.05). Superoxide dismutase (SOD), lysozyme (LZM), and acid phosphatase (ACP) activities in S3 and S4 groups were significantly higher than in S1, S2, and S5 groups (P < 0.05). Under these experimental conditions, dietary silymarin improved growth performance, reduced crude fat (EE) content, and enhanced non-specific immunity in Pelteobagrus fulvidraco, with the optimal supplementation level identified as 100 mg/kg.
[1] Pradhan S C, Girish C. Hepatoprotective herbal drug, silymarin from experimental pharmacology to clinical medicine[J]. Indian Journal of Medical Research, 2006, 124(5): 491-504.
[2] E. M A, F. A A A, E. A M E A, et al.MicroRNA-122 expression in hepatotoxic and γ-irradiated rats pre-treated with naringin and silymarin[J]. Journal of Radiation Research and Applied Sciences, 2020, 13(1): 47-55.
[3] 韩明, 文萍, 文剑波, 等. 水飞蓟素联合辛伐他汀治疗非酒精性脂肪性肝炎临床疗效观察[J]. 中国肝脏病杂志(电子版), 2011, 3(01): 15-18.
[4] Breschi M C, Martinotti E, Apostoliti F, et al.Protective effect of silymarin in antigen challenge- and histamine-induced bronchoconstriction in in vivo guinea-pigs[J]. European Journal of Pharmacology, 2002, 437(1-2): 91-95.
[5] Hasanthi M, Jo S , Kim H S, et al.Dietary supplementation of micelle silymarin enhances the antioxidant status, innate immunity, growth performance, resistance against Vibrio parahaemolyticus infection, and gut morphology in Pacific white shrimp (Litopenaeus vannamei)[J]. Animal Feed Science and Technology, 2024, 311.
[6] Chaklader M R, Ahmed H A, Khafaga A F, et al.Silybum marianum promotes growth, hepatic antioxidative activity, and splenic immunity but does not influence the intestinal barrier function of Nile tilapia, Oreochromis niloticus[J]. Aquaculture, 2024:583.
[7] Xiao P, Ji H, Ye Y, et al.Dietary silymarin supplementation promotes growth performance and improves lipid metabolism and health status in grass carp (Ctenopharyngodon idellus) fed diets with elevated lipid levels[J]. Fish Physiology & Biochemistry, 2017, 43(1): 245-263.
[8] Wang J, Zhou H, Wang X, et al.Effects of silymarin on growth performance, antioxidant capacity and immune response in turbot (Scophthalmus maximus L.)[J]. Journal of the World Aquaculture Society, 2019(6).
[9] Se H K, Sehee J, Sik K Y, et al.Effects of dietary micelle silymarin on the growth performance, feed utilization and health of olive flounder (Paralichthys olivaceus)[J]. Aquaculture International, 2023, 31(6): 3419-3436.
[10] 杨景峰, 包华驹, 张成国. 池塘主养黄颡鱼商品鱼高产试验与示范研究[J]. 现代农业科技, 2013(04): 278+280.
[11] 代云云, 袁永明, 袁媛, 等. 我国黄颡鱼产业生产现状与发展趋势分析[J]. 现代商贸工业, 2019, 40(23): 23-24.
[12] 孙越. 浅谈黄颡鱼养殖技术[J]. 沈阳教育学院学报, 2010, 12(02): 98-100.
[13] Qianqian Z, Min A J, Ho K I. Micelle silymarin supplementation to sows' diet from day 109 of gestation to entire lactation period enhances reproductive performance and affects serum hormones and metabolites[J]. Journal of Animal Science, 2021(12): 12.
[14] Wei L, Wu P, Zhou X Q, et al. Dietary silymarin supplementation enhanced growth performance and improved intestinal apical junctional complex on juvenile grass carp (Ctenopharyngodon idella)[J]. Aquaculture, 2020, 525(4): 735311.
[15] Yao, ChuanweiHuang, WenxingLiu, YongtaoYin, ZhaoyangXu, NingHe, YuliangWu, XiongfeiMai, KangsenAi, Qinghui.Effects of dietary silymarin (SM) supplementation on growth performance, digestive enzyme activities, antioxidant capacity and lipid metabolism gene expression in large yellow croaker (Larimichthys crocea) larvae[J]. Aquaculture Nutrition, 2020, 26(6).
[16] El-Haroun E R, Hassaan M S, Mohammady E Y, et al. Effect of Silybum marianum seeds as a feed additive on growth performance, serum biochemical indices, antioxidant status, and gene expression of Nile tilapia, Oreochromis niloticus (L.) fingerlings[J].Aquaculture, 2019, 509:178-187.
[17] 苟庚午, 蒋明, 文华, 等. 饲料中添加水飞蓟素对吉富罗非鱼生长性能、肝脏脂肪代谢酶和抗氧化能力的影响[J]. 水产学报, 2016, 40(09): 1309-1320.
[18] Ighodaro O M, Akinloye O A. First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): Their fundamental role in the entire antioxidant defence grid[J].Alexandria Journal of Medicine, 2017:S2090506817301550.
[19] 郑宗林, 王广军, 朱成科, 等. 水飞蓟素对黄曲霉毒素B1致毒的红姑鱼幼鱼生长和毒素积累的影响[J]. 中国畜牧杂志, 2016, 52(03): 50-55.
[20] 冷卓颖, 蔡博生, 吴天利, 等. 低盐度下金钱鱼对嗜水气单胞菌感染的生理生化及免疫响应[J/OL]. 海南热带海洋学院学报, 1-13[2025-02-23]. http://kns--cnki--net--https.cnki.mdjsf.utuvpn.utuedu.com:9000/kcms/detail/46.1085.G4.20250108.1545.006.html.
[21] Li T, Yan X, Xiao‐hui Dong, et al.Effects of choline supplementation on growth performance, liver histology, nonspecific immunity and related genes expression of hybrid grouper (♀ Epinephelus fuscoguttatus × ♂ E. lanceolatu) fed with high-lipid diets. [J]. Fish & shellfish immunology, 2023:, 108815.
[22] Chen Y, Huang X, Wang J, Li C. Effect of pure microcystin-LR on activity and transcript level of immune-related enzymes in the white shrimp (Litopenaeus vannamei). Ecotoxicology. 2017 Jul; 26(5): 702-710. doi: 10. 1007/s10646-017-1802-7. Epub 2017 May 2. PMID: 28466205.
[23] Saurabh S, Sahoo P K. Lysozyme: an important defence molecule of fish innate immune system[J]. Aquaculture Research, 2010, 39.
[24] Kong X, Wang S, Jiang H, et al.Responses of acid/alkaline phosphatase, lysozyme, and catalase activities and lipid peroxidation to mercury exposure during the embryonic development of goldfish Carassius auratus[J]. Aquatic Toxicology, 2012, 120-121(none): 119-125.
[25] Abdel-Latif H M R, Shukry M, Noreldin A E, et al.Milk thistle (Silybum marianum) extract improves growth, immunity, serum biochemical indices, antioxidant state, hepatic histoarchitecture, and intestinal histomorphometry of striped catfish, Pangasianodon hypophthalmus[J]. Aquaculture, 2023.
[26] 贾睿, 曹丽萍, 杜金梁, 等. 水飞蓟素对四氯化碳致鲫肝(细胞)损伤的保护和抗氧化作用[J]. 中国水产科学, 2013, 20(03):551-560.
[27] 李会峰, 李二超, 徐畅, 等. 饲料中添加水飞蓟素对低盐度下凡纳滨对虾生长、免疫、肝胰腺组织结构及肠道菌群的影响[J]. 水产学报, 2021, 45(01):98-114.
[28] 黄彩玲, 肖柳英, 潘竞锵. 水飞蓟素对小鼠肝损伤保护作用及机理研究[J]. 中国民族民间医药杂志, 2012.
[29] YU Z G, WU F, TIAN J, et al.Protective effects of compound ammonium glycyrrhizin,L-arginine,silymarin and glucurolactone against liver damage induced by ochratoxin a in primary chicken hepatocytes[J]. Molecular Medicine Reports, 2018, 18(3): 2551-2560.
基本信息:
中图分类号:S965.199
引用信息:
[1]尹思玲,卢璐,周芷多,等.水飞蓟素对黄颡鱼生长性能、体组成及非特异性免疫指标的影响[J].经济动物学报().
基金信息:
吉林省科技发展计划项目(20240303067NC); 国家特色淡水鱼产业技术体系(CARS-46); 吉林省教委科研基金(JKH20230412KJ)
2025-10-29
2025-10-29
2025-10-29