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肝脏是家禽体内重要的代谢器官承担着多种功能，其发育和脂肪合成是一个由多种信号分子及通路共同调节且复杂而精密的过程。STAT3是多种细胞信号通路的关键调节因子，影响细胞增殖、分化、凋亡及脂肪合成等过程，但其在家禽肝脏中的调控机制尚不明晰。本研究基于基因表达模式研究，发现鹅胚胎肝脏中STAT3呈现阶段特异性表达，进一步通过胚蛋注射STAT3抑制剂Stattic揭示其双重功能机制。结果表明，STAT3活性抑制以剂量依赖性方式显著降低肝细胞脂滴沉积（P < 0.05），并伴随脂质合成关键酶FASN和ACC1基因表达下调，证实STAT3通过调控脂肪酸生物合成通路影响脂肪代谢。同时，STAT3磷酸化水平受Stattic浓度梯度抑制后，引发细胞周期调控失衡，表现为增殖相关蛋白表达降低而凋亡标志物上调，形成“增殖抑制－凋亡激活”的分子网络。综上，本研究表明STAT3通过协调脂质合成与细胞稳态的双重机制，在鹅肝脏发育中发挥核心调控作用，为禽类肝脏发育生物学提供了新的分子机制视角。
Abstract：The liver is a vital metabolic organ in poultry， performing diverse functions. Its development and lipid synthesis constitute a complex and precisely regulated process mediated by multiple signaling molecules and pathways. As a key regulator of various cellular signaling pathways， STAT3 influences processes including cell proliferation， differentiation， apoptosis， and lipogenesis. However， its regulatory mechanisms in avian liver remain unclear. This study investigated STAT3's stage-specific expression pattern in goose embryonic liver through gene expression profiling and revealed its dual functional mechanisms via in ovo injection of STAT3 inhibitor Stattic. Results demonstrated that STAT3 inhibition significantly reduced hepatocyte lipid droplet deposition in a dose-dependent manner (P < 0.05)， accompanied by downregulation of lipid synthesis key enzymes FASN and ACC1 gene expression， confirming STAT3's regulatory role in fatty acid biosynthesis pathway. Concurrently， concentration-dependent suppression of STAT3 phosphorylation disrupted cell cycle regulation， manifested by decreased proliferation-related proteins and upregulated apoptotic markers， forming a "proliferation inhibition-apoptosis activation" molecular network. Collectively， this study reveals that STAT3 exerts central regulatory functions in goose liver development through dual mechanisms coordinating lipid synthesis and cellular homeostasis， providing novel molecular insights into avian hepatic developmental biology.
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基本信息:
中图分类号:S835
引用信息:
[1]侯嘉惠,胡湘曼,周宇轩,等.STAT3基因在鹅胚肝脏发育过程中的功能[J].经济动物学报().
基金信息:
吉林省自然科学基金项目（202401012241C）; 吉林省科技发展计划重点研发项目（20230202064NC）; 吉林省畜牧业管理局畜禽养殖成熟技术集成应用与示范推广项目（2025）
2025-06-27
2025-06-27
2025-06-27