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为了分析马鹿鞭肽的氨基酸组成和分子质量分布，探究马鹿鞭肽对性功能障碍大鼠的改善作用。利用SD大鼠腹腔注射环磷酰胺（Cyclophosphamide， CTX）建立性功能障碍模型，通过灌胃西地那非和马鹿鞭肽评价大鼠体重和脏器指数，检测大鼠血清睾酮（Testosterone， T）、促黄体生成素（Luteinizing hormone， LH）和一氧化氮（Nitric oxide， NO）水平，分析大鼠肾脏和睾丸组织病理变化情况。结果表明：马鹿鞭肽富含多种氨基酸，其中精氨酸的含量较多；其在1000 u以下的分子量段含量为68.92%；在功能方面，模型组与空白对照组相比，大鼠体重、肾脏、睾丸和胸腺指数以及T、LH和NO水平均下降，肾脏和睾丸组织发生明显病变，而低剂量和高剂量的马鹿鞭肽处理组大鼠的上述指标均有不同程度改善。马鹿鞭肽营养价值较高，产物以短链多肽为主，且能有效提高机体脏器指数、T、LH以及NO水平，缓解肾脏和睾丸病变，从而达到改善性功能的作用。
Abstract：To analyze the amino acid composition and molecular mass distribution of Cervus elaphus and explore the improvement effect of Cervus elaphus on sexual dysfunction rats. SD rats were injected with cyclophosphamide to establish sexual dysfunction model. After intragastric administration of sildenafil and Cervus elaphus peptide， the body weight， organ index， serum levels of testosterone， luteinizing hormone and nitric oxide were detected， and pathological changes of kidney and testis were analyzed. Cervus elaphus peptide is rich in many amino acids， among which the content of arginine is more; the content of molecular weight segment below 1000 Da is 63.59%. In terms of function， compared with the blank control group， the body weight， kidney， testicular and thymus indexes， T， LH and NO levels of rats were decreased in the model group， and the kidney and testicular tissue lesions were obvious， while the above indexes of rats in the low-dose and high-dose. Cervus elaphus peptide treatment groups were improved to varying degrees. We can konw that Cervus elaphus peptide has high nutritional value， and the products are mainly short-chain polypeptides， which can effectively improve the organ index， T， LH and NO levels， alleviate kidney and testicular lesions， and thus improve sexual function. The results of this study can provide a reference for the exploration of mechanism of Cervus elaphus peptide in improving sexual dysfunction and the application of Cervus elaphus peptide in functional foods.
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基本信息:
中图分类号:R285.5
引用信息:
[1]郑丽萍,熊菲菲,孙艺,等.马鹿鞭肽对环磷酰胺致大鼠性功能障碍的改善作用研究[J].经济动物学报().
基金信息:
中国轻工集团科技创新基金项目（ZQ2023JC-SW04)
2024-12-03
2024-12-03
2024-12-03