| 67 | 0 | 2 |
| 下载次数 | 被引频次 | 阅读次数 |
分子生物学的快速发展推动动物育种进入分子层面,但我国犬重要性状分子标记研究与应用远滞后于其他畜禽。本文梳理分子标记检测技术,综述犬繁殖、生长、抗逆性状相关分子标记研究进展,探讨当前犬分子标记研究的不足与应用前景,以期为警用等功能型犬种的分子育种提供参考。
Abstract:Molecular biology has enabled animal breeding at the molecular level. In China, however, molecular marker studies of key canine traits were initiated later than in other livestock and remain scarcely applied. Here we systematically review common marker techniques and critically summarize progress in mapping markers for canine reproductive, growth, and stress resistance traits. Current bottlenecks and future prospects are discussed to provide a reference for the molecular breeding of specialized working dogs, such as police and service animals.
[1] Yuan Y, Bayer PE, Batley J, et al. Current status of structural variation studies in plants[J]. Plant Biotechnology Journal, 2021, 19(11): 2153-2163.
[2] 彭佩雅,陈钰焓,杨龙,等. 家畜基因组拷贝数变异研究进展[J]. 畜牧兽医学报, 2024, 55 (04): 1356-1369.
[3] 陈晓敏,李沂霖,何炜诺,等. 分子标记技术在山羊育种中的研究进展[J]. 中国草食动物科学, 2023, 43(06): 45-51.
[4] Laufer V A, Glover T W, Wilson T E. Applications of advanced technologies for detecting genomic structural variation[J]. Mutation Research-Reviews in Mutation Research, 2023, 792:108475.
[5] Wang G D, Zhai W, Yang H C, et al. Out of southern East Asia: the natural history of domestic dogs across the world[J]. Cell Research, 2015, 26(1): 21–33.
[6] Zhang Z, Khederzadeh S, Li Y. Deciphering the puzzles of dog domestication[J]. Zoological Research, 2020, 41(2): 97–104.
[7] Hasan N, Choudhary S, Naaz N, et al. Recent advancements in molecular marker-assisted selection and applications in plant breeding programmes[J]. Journal of Genetic Engineering and Biotechnology, 2021, 19(1): 128.
[8] 杨青青, 唐家琪, 张昌泉, 等. KASP标记技术在主要农作物中的应用及展望[J]. 生物技术通报, 2022, 38(4): 58–71.
[9] Hu W, Zhou T, Wang P, et al. Development of whole-genome agarose-resolvable LInDel markers in Rice[J]. Rice (New York, N.Y.), 2020, 13(1): 1.
[10] 李其琴. 三江源国家公园长江源园区藏原羚(Procapra picticaudata)遗传多样性及亲缘关系研究[D]. 烟台: 烟台大学, 2025.
[11] 罗春彦,白锋,周喜荣,等. 微卫星标记技术在绵羊育种中的应用[J]. 中国畜牧杂志, 2024, 60(7): 54-59.
[12] Yu R, Chen X, Zhang H, Zhang Q, et al. Development of SSR markers related to salinity resistance based on transcriptomic sequences in Medicago sativa[J]. PLoS One, 2025, 20(11): e0336528.
[13] 王攀. 植物分子标记高通量快速检测技术的研究进展[J]. 中国种业, 2024, (7): 17-22.
[14] Park J S, Choi Y, Kim J H, et al. Development of a web-based high-throughput marker design program: CAPS (cleaved amplified polymorphic sequence) Maker[J]. Plant Methods. 2024, 20(1): 192.
[15] 倪佳俊,韩冉,徐文竞,等. 希尔斯山羊草1S~(s)S和3S~(s)S染色体特异dCAPS标记的建立[J]. 山东农业科学, 2023, 55(5): 15-21.
[16] Ma R, Lu Y, Li M, et al. Whole-genome resequencing in sheep: Applications in breeding, evolution, and conservation[J]. Genes (Basel), 2025, 16(4): 363.
[17] Hu T, Chitnis N, Monos D, et al. Next-generation sequencing technologies: An overview [J]. Human Immunology, 2021, 82(11): 801–811.
[18] Lu Y, Li M, Gao Z, et al. Advances in whole-genome sequencing: Methods, Tools, and Applications in Population Genomics[J]. International Journal of Molecular Sciences, 2025, 26(1): 372.
[19] 王琦, 朱迪, 王宇哲, 等. 全基因组SNP分型策略及基因组预测方法的研究进展[J]. 畜牧兽医学报, 2020, 51(2): 205-216.
[20] Reyes V P, Kitony J K, Nishiuchi S, et al. Utilization of genotyping-by-sequencing (GBS) for Rice pre-breeding and improvement: A Review[J]. Life (Basel). 2022, 12(11): 1752.
[21] Rajendran N R, Qureshi N, Pourkheirandish M. Genotyping by sequencing advancements in Barley[J]. Frontiers in Plant Science, 2022, 13: 931423.
[22] Adler A J, Wiley G B, Gaffney P M. Infinium assay for large-scale SNP genotyping applications[J]. Journal of Visualized Experiments : JoVE, 2013, (81): e50683.
[23] 王有栋,曹志平,李玉茂,等.SNP芯片技术原理及其在鸡遗传育种中的应用[J].畜牧兽医学报,2025,56(9):4165-4175.
[24] Van Asselt A J, Ehli E A. Whole-genome genotyping using DNA microarrays for population genetics[J]. Methods in Molecular Biology (Clifton, N.J.), 2022, 2418: 269-287.
[25] Guo Z, Yang Q, Huang F, et al. Development of high-resolution multiple-SNP arrays for genetic analyses and molecular breeding through genotyping by target sequencing and liquid chip[J]. Plant Communications, 2021, 2(6): 100230.
[26] Wang J, Xing S, Zhou Y, et al. Research note: A low-density SNP genotyping panel for Chinese native chickens[J]. Poultry Science, 2025, 104(1): 104609.
[27] Wang H, Wu H, Zhang W, et al. Development and validation of a 5K low-density SNP chip for Hainan cattle[J]. BMC Genomics, 2024, 25(1): 873.
[28] Dipta B, Sood S, Mangal V, et al. KASP: a high-throughput genotyping system and its applications in major crop plants for biotic and abiotic stress tolerance[J]. Molecular Biology Reports, 2024, 51(1): 508.
[29] Liu C, He Y, Liang W, et al. Research Note: Development and application of specific molecular identity cards for “Yufen 1” H line chickens[J]. Poultry Science, 2024, 103(2): 103343.
[30] Zheng W, Wu P, Zhu M, et al. Establishment and validation of a method for the identification of recessive mastitis resistance Genes in dairy cows[J]. Genes, 2025, 16(5): 485.
[31] Marelli S P, Beccaglia M, Bagnato A, et al. Canine fertility: The consequences of selection for special traits[J]. Reproduction in Domestic Animals, 2020, 55(S2): 4–9.
[32] 李静, 万九生, 陈超, 等. 警犬繁殖性状的遗传参数评估[J]. 安徽农业科学, 2022, 50(2): 95–98.
[33] Mostert B E, Marle-Koster E V, Visser C, et al. Genetic analysis of pre-weaning survival and inbreeding in the Boxer dog breed of South Africa [J]. South African Journal of Animal Science, 2015, 45(5): 476–484.
[34] Smith S P, Phillips J B, Johnson M L, et al. Genome-wide association analysis uncovers variants for reproductive variation across dog breeds and links to domestication[J]. Evolution, Medicine, and Public Health, 2019, 2019(1): 93–103.
[35] ?nan? M E, Tekin K, Akkurt M Y, et al. Genomewide association of male reproductive traits in Aksaray Malakli dogs[J]. Reproduction in Domestic Animals, 2018, 53(6): 1555–1562.
[36] 李涛, 马长书, 魏荣兴, 等. BLUP法在警用罗威纳犬体尺遗传参数计算中的应用[J]. 黑龙江畜牧兽医, 2016, (10): 181–182.
[37] Haque M A, Kim N K, Yeji R, et al. Genomic prediction and genome-wide association studies of morphological traits and distraction index in Korean Sapsaree dogs[J]. PLoS One, 2024, 19(11): e0312583.
[38] Sheet S, Kim J S, Ko M J, et al. Insight into the candidate genes and enriched pathways associated with height, length, length to height ratio and body-weight of Korean indigenous breed, Jindo dog using gene set enrichment-based GWAS analysis[J]. Animals (Basel), 2021, 11(11): 3136.
[39] 韦云芳,李飞翔,彭建国,等.昆明犬IGF1R基因外显子多态性与体重和体尺性状的关联性分析[J]. 黑龙江畜牧兽医, 2024, (12): 115-121.
[40] 韦云芳,李飞翔,汪斌,等. 昆明犬IGF1基因在肝脏中的表达特征及遗传多样性分析 [J]. 黑龙江畜牧兽医, 2024, (04): 108-114.
[41] Plassais J, Rimbault M, Williams F J, et al. Analysis of large versus small dogs reveals three genes on the canine X chromosome associated with body weight, muscling and back fat thickness[J]. PLoS genetics, 2017, 13(3): e1006661.
[42] Plassais J, Kim J, Davis B W, et al. Whole genome sequencing of canids reveals genomic regions under selection and variants influencing morphology[J]. Nature communications, 2019, 10(1): 1489.
[43] Sheet S, Krishnamoorthy S, Cha J, et al. Identification of candidate genes and pathways associated with obesity-related traits in canines via gene-set enrichment and pathway-based GWAS analysis[J]. Animals (Basel), 2020, 10(11): 2071.
[44] Mankowska M, Stachowiak M, Graczyk A, et al. Sequence analysis of three canine adipokine genes revealed an association between TNF polymorphisms and obesity in Labrador dogs[J]. Animal Genetics, 2015, 47(2): 245–249.
[45] Sypniewski M, Szydlowski M. A study of 41 canine orthologues of human genes involved in monogenic obesity reveals marker in the ADCY3 for body weight in Labrador Retrievers[J]. Veterinary sciences , 2023, 10(6): 390.
[46] Wang G D, Fan R X, Zhai W, et al. Genetic convergence in the adaptation of dogs and humans to the high-altitude environment of the tibetan plateau[J]. Genome Biology and Evolution, 2014, 6(8): 2122–2128.
[47] Gou X, Wang Z, Li N, et al. Whole-genome sequencing of six dog breeds from continuous altitudes reveals adaptation to high-altitude hypoxia[J]. Genome Research, 2014, 24(8): 1308–1315.
[48] Signore A V, Yang Y Z, Yang Q Y, et al. Adaptive changes in hemoglobin function in high-altitude tibetan canids were derived via gene conversion and introgression[J]. Molecular Biology and Evolution, 2019, 36(10): 2227–2237.
[49] Wu H, Liu Y H, Wang G D, et al. Identifying molecular signatures of hypoxia adaptation from sex chromosomes: a case for Tibetan Mastiff based on analyses of X chromosome[J]. cientific Reports, 2016, 6(1): 35004.
[50] Yang Q, Chen H, Ye J, et al. Genetic diversity and signatures of selection in 15 chinese indigenous dog breeds revealed by genome-wide SNPs[J]. Frontiers in Genetics, 2019, 10(1): 1174.
[51] Liu Y H, Wang L, Xu T, et al. Whole-genome sequencing of african dogs provides insights into adaptations against tropical parasites[J]. Molecular Biology and Evolution, 2018, 35(2): 287–298.
[52] 柳延虎. 全基因组水平探究家犬温度适应的遗传机制[D]. 昆明: 云南大学, 2018.
基本信息:
中图分类号:S829.2
引用信息:
[1]宋珍全,徐兆云,刘成功.分子标记在犬繁殖、生长和抗逆性状研究中的应用[J].经济动物学报().
基金信息:
国防科技创新项目(ZZKY20253109)
2025-12-18
2025-12-18
2025-12-18