经济动物学报

为探究不同盐度急性胁迫对陆封型马苏大麻哈幼鱼肝胰脏功能及抗氧化能力的影响，在实验室条件下设置1个对照组和5个盐度组，分别记为G0(0.2‰)、G1(4.7‰)、G2(8.8‰)、G3(13.1‰)、G4(17.4‰)、G5(21.2‰)并设置五重复。每试验缸放30尾幼鱼，平均体长(7.9±0.8)cm，平均体质量(3.79±1.44)g。试验后0 h、12 h、24 h、48 h、72 h和96 h分别取样，测定幼鱼肝胰脏中GPT、GOT、GSH-Px酶活性及TP、MDA含量。试验结果显示，该幼鱼经不同盐度急性胁迫后各组别肝胰脏中TP均有不同提升，显著高于0 h(P<0.05)，盐度越高，TP水平越高(P<0.05)且各组差异性显著(P＜0.05)。GPT和GOT酶活性都有所降低趋势，各组差异性显著(P<0.05)，且在(G4-G5)组中GOT酶活性降低幅度大于GOT。GSH-Px酶活性呈现先升高后降低的趋势。MDA含量在(G1-G3)组变化不大，且三组间差异不显著(P＞0.05)，G4组略有升高，G5组显著升高。研究结果有助于进一步了解在受到急性盐度胁迫状态下，陆封型马苏大麻哈1龄⁺幼鱼肝胰脏受损程度和抗氧化能力应答，从而为探究通过增殖放流陆封型马苏大麻哈幼鱼实现增加海洋中马苏大麻哈鱼种群的可能性提供数据参考。【结论】陆封型马苏大麻哈1龄⁺幼鱼在降海过程适宜盐度为0.2‰-8.8‰。

To investigate the effects of acute salinity stress at different levels on the hepatic-pancreatic function and antioxidant capacity of landlocked masu salmon (Oncorhynchus masou) juveniles， five salinity groups were set up under laboratory conditions: G1(4.7‰)， G2(8.8‰)， G3(13.1‰)， G4(17.4‰)， and G5(21.2‰)， with five replicates each. Each experimental tank contained 30 juveniles with an average body length of (7.9±0.8) cm and an average body weight of (3.79±1.44) g. Samples were taken at 0 h， 12 h， 24 h， 48 h， 72 h and 96 h after the experiment to determine the activities of GPT， GOT， GSH-Px enzymes and the contents of TP and MDA in the hepatopancreas of juvenile fish. The experimental results showed that the TP in the hepatopancreas of each group of juvenile fish increased differently after acute salinity stress， which was significantly higher than that at 0 h (P<0.05). The higher the salinity， the higher the TP level (P<0.05)， and the differences among groups were significant (P＜0.05). The activities of GPT and GOT enzymes showed a decreasing trend， and the differences among groups were significant (P<0.05). Groups G4-G5， the decrease range of GOT enzyme activity was greater than that of GOT. The activity of GSH-Px enzyme showed a trend of first increasing and then decreasing. The MDA content in groups G1-G3 changed little， and there was no significant difference among the three groups (P＞0.05). The G4 group increased slightly， and the G5 group increased significantly. The research results help to further understand the damage degree of the hepatopancreas and the antioxidant capacity response of landlocked masu salmon over 1-year-old juvenile fish under acute salinity stress， so as to provide data reference for exploring the possibility of increasing the population of masu salmon in the ocean through the stocking of landlocked masu salmon juvenile fish. [Conclusion] The suitable salinity for landlocked masu salmon over 1-year-old juvenile fish during the seaward migration is 0.2‰-8.8‰.