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为探究纳米肉碱对牛冷冻精液品质的调控作用，本研究评估了稀释液中添加不同浓度纳米肉碱（0、5μg/mL、10μg/mL、20μg/mL、40μg/mL）对冻融后精液品质的影响。试验通过检测冻融后精子活力、运动学参数、顶体完整率及精浆氧化损伤与抗氧化酶活性，分析纳米肉碱的作用效果。结果表明：与对照组相比，10μg/mL、20μg/mL纳米肉碱显著提升冻后精子活力（P＜0.01），并改善运动学参数，包括平均路径距离（DAP）、直线距离（DSL）、平均路径速度（VAP）、曲线速度（VCL）等；5μg/mL、10μg/mL、20μg/mL纳米肉碱可显著提高精子顶体完整率（P＜0.05或P＜0.01），并降低精浆丙二醛（MDA）含量（P＜0.05或P＜0.01），其中10μg/mL、20μg/mL组超氧化物歧化酶（SOD）活性显著增强（P＜0.05或P ＜0.01）。研究表明，纳米肉碱通过提升抗氧化能力，减轻冷冻损伤来改善牛精子结构完整性与运动性能，且以10μg/mL、20 μg/mL浓度效果最佳。
Abstract：Abstact: In order to investigate the modulatory effect of nano-carnitine on the quality of bovine frozen semen， the present study evaluated the effect of adding different concentrations of nano-carnitine (0， 5μg/mL， 10μg/mL， 20μg/mL and 40 μg/mL) to the diluent on the quality of spermatozoa after freezing and thawing. The experiment was conducted to analyze the effect of nano-carnitine by detecting sperm viability， kinematic parameters， acrosome integrity， and seminal plasma oxidative damage and antioxidant enzyme activities after freezing and thawing. The results showed that 10 and 20 μg/mL nano-carnitine significantly enhanced post-freezing sperm viability (P < 0.01) and improved the kinematic parameters， including the mean distance of path (DAP)， distance to straight line (DSL)， mean velocity of path (VAP)， and velocity of curves (VCL)， compared with the control group; and 5μg/mL， 10μg/mL and 20 μg/mL nano-carnitine significantly increased the acrosome integrity rate of spermatozoa ( P ＜0.05 or P ＜0.01) and reduced seminal plasma malondialdehyde (MDA) content (P ＜0.05 or P ＜0.01)， in which superoxide dismutase (SOD) activity was significantly enhanced in the 10 μg/mL and 20 μg/mL groups (P ＜0.05 or P ＜0.01). The study showed that nano-carnitine improved the structural integrity and motility of spermatozoa by enhancing the antioxidant capacity and reducing the freezing damage， and the best effect was observed at the concentration of 10 μg/mL and 20 μg/mL.
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基本信息:
中图分类号:S823
引用信息:
[1]侯春琦,郭乐薇,朱清玉,等.纳米肉碱对牛冷冻精液品质及抗氧化能力的影响[J].经济动物学报().
基金信息:
吉林省肉牛产业化发展重大科技专项（YDZ202203CGZH041）; 肉用种公牛精液品质性状形成机制研究（0205202020141）
2025-12-12
2025-12-12
2025-12-12