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目的 分析北京市朝阳区流腮样病例和流感样病例中人副流感病毒3(Human parainfluenza virus type 3,HPIV-3)流行情况及其血凝素-神经氨酸酶(Hemagglutinin-neuraminidase, HN)基因特征。方法 对北京市朝阳区2023-2024年251份流腮样病例腮腺管口拭子标本和5618份流感样病例咽拭子标本进行HPIV-3实时荧光PCR检测，应用反转录聚合酶链反应(Reverse transcription polymerase chain reaction, RT-PCR)方法扩增阳性标本的HN基因并对扩增产物进行测序，然后与GenBank数据库中的国内外HPIV-3 HN基因进行对比分析。结果 两种来源共计5869份标本中筛查出HPIV-3阳性165份，检出高峰为春、夏两季。HPIV-3检出率流腮样病例组为17.53%(44/251)，流感样病例组为2.15%(121/5618)。两组病例HPIV-3检出率差异有统计学意义(x2=72.97,P<0.05)。阳性患者平均年龄流腮样病例组为7.29岁，流感样病例组为17.28岁，两组HPIV-3阳性患者年龄平均值差异有统计学意义(t=2.64,P=0.01)。通过RT-PCR方法扩增获得37株HPIV-3 HN基因全长序列，毒株间核苷酸和氨基酸一致性分别为91.71%～100.0%和95.1%～100.0%。其中34株属于C3亚型(15株C3a,1株C3b,18株C3f),2株属于C5亚型，1株为D基因型。两病例组间C3a和C3f分布无差异。结论 HPIV-3是北京市朝阳区流腮样病例和流感样病例中检出的病原体之一，检出高峰为春、夏季。在流腮样病例中感染人群集中在儿童青少年。HPIV-3HN核苷酸序列和氨基酸序列均具有高度的一致性，C3a,C3f,C3b,C5进化分支在北京市朝阳区共流行，并以C3a、C3f占为主。C3a、C3f在两种病例样本中分布差异无统计学意义。在北京地区首次发现D基因型HPIV-3。
Abstract：Objective To analyze the epidemiological characteristics of human parainfluenza virus type 3(HPIV-3) in mumps-like and influenza-like cases in Chaoyang District, Beijing, during 2023– 2024, and to characterize the hemagglutinin – neuraminidase(HN) gene of circulating strains. Methods A total of 251 parotid duct swab specimens from mumps-like cases and 5618 throat swab specimens from influenza-like cases collected in Chaoyang District, Beijing, China, during 2023–2024 were tested for HPIV-3 using real-time RTPCR. The HN gene of positive samples was amplified by reverse transcription polymerase chain reaction(RTPCR), and the PCR products were sequenced. The obtained sequences were compared with reference HPIV-3 HN sequences retrieved from GenBank, and phylogenetic analysis was performed. Results Among 5869 specimens from the two case populations, 165 were positive for HPIV-3, with peak detection occurring in spring and summer. The detection rate was significantly higher in the mumps-like case group(17.53%, 44/251) than in the influenza-like case group(2.15%, 121/5618) (χ2 = 72.97, P < 0.05). The mean age of HPIV-3–positive patients was 7.29 years in the mumps-like group and 17.28 years in the influenza-like group, and the difference was statistically significant(t = 2.64, P = 0.01). Thirty-seven full-length HN gene sequences were successfully obtained. Nucleotide and amino acid identities among the strains ranged from 91.71% to 100.0% and from 95.1% to 100.0%, respectively. Phylogenetic analysis showed that 34 strains belonged to genotype C3(including 15 C3a, 1 C3b, and 18 C3f), two strains to genotype C5, and one strain to genotype D. No significant difference was observed in the distribution of C3a and C3f between the two case groups. Conclusion HPIV-3 is one of the pathogens detected in both mumps-like and influenza-like cases in Chaoyang District, Beijing, with peak circulation occurring in spring and summer. In mumps-like cases, infections are predominantly observed in children and adolescents. The HN gene sequences exhibit high nucleotide and amino acid identity among circulating strains. Genotypes C3a, C3f, C3b, and C5 co-circulate locally, with C3a and C3f predominating, and no significant difference in genotype distribution is observed between the two case populations. Genotype D HPIV-3 was identified in Beijing for the first time.
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基本信息:
中图分类号:R511.7;R181.3
引用信息:
[1]高艳,张士尧,陈昱达,等.北京市朝阳区2023-2024年两种病例来源人副流感病毒3型流行特征和基因型别研究[J].病毒学报().
基金信息:
北京“高创计划-登峰”项目(项目号:G202513075)
2026-05-07
2026-05-07
2026-05-07